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Identification of Phytophthora Species Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年05
Page:
113-119
Research Field:
Publishing date:

Info

Title:
Identification of Phytophthora Species Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes
Author(s):
WANG Jie-feiCAO Zhi-minBAI Lu-chaoTANG YiZHOU Xue
(College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100)
Keywords:
pricklyashPhytophthoramorphologyrDNA-ITSβ-tubulinpathogenicityantagonism
PACS:
-
DOI:
10.11937/bfyy.201505034
Abstract:
Taking species and distribution of Phytophthora which were isolated from pricklyash (Zanthoxylum bungeanum) in Shaanxi and Gansu provices as material,based on colony,sporangium and oogonium morphology,ITS and β-tubulin gene sequences and their pathogenicity,species and distribution of the bacteria were studied,and the antagonistic bacteria of pricklyash Phytophthora were screened.The results showed that Phytophthora isolates Pfs3,Pfs4,Pbjt and Pfs5 from Baoji of Shaanxi were identified as Phytophthora boehmeriae Sawada,and isolates Pcsx,Pwmc,Pwqh,Pwbs3 and Pwbs2 from Longnan of Gansu were identified as Phytophthora multivora Scott and Jung.One actinomycetes isolated from the soil of pricklyash field was screened out,and preliminary identified as Streptomyces sp.which showed antagonistic activity to these Phytophthora species.

References:

 

[1] 孟铁男,冯泽,洪流,.花椒黑胫病的发生及防治技术研究[J].甘肃农业科技,1993(2)31-33.

[2] 雷家瑶,车兴璧,李锋.甘肃省花椒黑胫病的研究初报[J].植物保护,1990(3)8-10.

[3] 何苏琴,袁忠林,洪流,等.花椒流胶病病原疫霉种的鉴定[J].西北农业学报,1997,6(4)4-7.

[4] 谢宁,曹支敏,梁超琼,.花椒疫霉病菌种的鉴定[J].西北林学院学报,2013,28(2)125-130.

[5] 余永年.中国真菌志(第六卷 霜霉目)[M].北京:科学出版社,1998123.

[6] Kirk P M,Cannonce P F,Minter D W,et al.Dictionary of the Fungi.(10th ed.) [M].UK CABI Europe-UK2008753.

[7] Cooke D E L,Drenth A,Duncan J Met al.A molecular phylogeny of Phytophthora and related Oomycetes[J].Fungal Genet Biol,2000,3017-32.

[8] Fster HCummings M PCoffey M D.Phylogenetic relationships of Phytophthora species based on ribosomal ITS I DNA sequence analysis with emphasis on Waterhouse groups V and VI[J].Mycol Res2000104(9)1055-1061.

[9] 张正光,郭宝成,王源超,.非洲菊根腐病病原的鉴定与ITS序列分析[J].植物病理学报,2005,35(5)392-396.

[10]燕勇,李卫平,高雯,等.rDNA-ITS序列分析在真菌鉴定中的应用[J].中国卫生检验杂志,2008,18(10)1958-1961.

[11]Blair J E,Coftey M D,Park S Y,et al.A multi-locus phylogeny for Phytophthora utilizing morkers derived from complete genome sequences[J].Fungal Genet Biol,2008,45:266-277.

[12]Da Vinha F N MGravina-Oliveira M PFranco M Net.al.Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic biomass as induce substrate[J].Appl Biochem Biotechnol2011164256-267.

[13]Kortemaa HPennanen TSmolander A,et al.Distribution of antagonistic Streptomyces griseoviridis in rhtzosphere and nonrhizosphere sand[J].Journal of Phytopathology1997,145(4)137-143.

[14]方中达.植病研究技术[M].北京:中国农业出版社,1998.

[15]孙广宇,宗兆峰.植物病理学实验技术[M].北京:中国农业出版社,2002.

[16]郑小波.疫霉菌及其研究技术[M].北京:中国农业出版社,1997.

[17]Kroon L PBrouwer Hde Cock A M,et al.The genus Phytophthora anno 2012[J].Phytopathology2012102(4)348-364.

[18]张颖慧,魏东盛,邢来君,.一种改进的丝状真菌DNA提取方法[J].微生物学通报,200835(3)466-469.

[19]易润华,朱西儒,周而勋.简化CTAB法快速微量提取丝状真菌DNA[J].湛江海洋大学学报,2003,23(6)72-73.

[20]White T J,Bruns TLee Set al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]// InInnis M AGelfand D HSninsky J Jet al.eds.PCR Protocols.A Guide to Methods and Applications New YorkAcademic Press,1990315-322.

[21]曹支敏,明玉玲,陈丹,.花椒干腐病的寄主抗病性与病原菌致病性分化[J].西北林学院学报,2010,25(6)115-118.

[22]沈萍,范秀荣,李广武.微生物学实验[M].北京:高等教育出版社,2005.

[23]王群利,师宝君,卞小莹,.土壤拮抗放线菌的分离及其抗菌活性筛选[J].植物保护,2008,34(3)41-46.

[24]Scott P M,Burgess T I,Barber P A,et al.Phytophthora multivora sp.nov.a new species recovered from declining EucalyptusBanksia,Agonis and other plant species in Western Australia [J].Persoonia2009221-13.

[25]姜玉,董怀玉,徐秀德,等.放线菌在植病生防中的研究进展[J].杂粮作物,200525(5)329-331.

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Last Update: 2015-06-24