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Establishment of Embryogenic Cell Suspension and Protoplast Culture of Hedychium coccineum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年05
Page:
104-108
Research Field:
Publishing date:

Info

Title:
Establishment of Embryogenic Cell Suspension and Protoplast Culture of Hedychium coccineum
Author(s):
XIAO Wang; TU Hong-yan; DENG Chong-hui
(Department of Biology，Guangdong University of Education，Guangzhou，Guangdong 510303)
Keywords:
Hedychium coccineum; embryogenic cell suspensions; protoplast
PACS:
-
DOI:
10.11937/bfyy.201505032
Abstract:
Embryogenci cell suspensions were established for the ornamental ginger Hedychium coccineum using filaments and anthers.Embryagenic indction,cell suspension culture system establishment and protolast culture of Hedychium coccineum were studied.The results showed that,after culture for 120 days，calli were induced on induced medium (Murashige and Skoog (MS) basal medium supplemented with 4 mg/L 2，4-D+4 mg/L NAA+1 mg/L 6-BA+30 g/L sucrose+7 g/L agar).The calli were then transferred on proliferated medium (MS basal medium+1 mg/L 2，4-D+0.25 mg/L NAA+0.25 mg/L 6-BA）and light yellow and friable embryogenic callus were obtained.These embryogenic callus were suspended in liquid medium，and after 3 months culture，a homogeneous and stable embryogenic cell suspension (ECS)，composed of small cell aggregates，was established.Viable protoplasts were isolated from ECS in a enzyme mixture of 3.0% (w/v) cellulose R-10，2% (w/v) macerozyme R-10 and 0.25% (w/v) pectinase Y-23 for 7 hours，enzyme solution with 0.14 mol/L manntitol resulted in the highest yield of 2.25×105 protoplasts per mL PCV ECS.Feeder layer culture systems were used for protoplast culture.Frequency of cell division at 14 days and colony formation at 28 days were 12.3% and 4.2% respectively.However，all protoplast-derived cell colonies could not develop further.

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Last Update: 2015-06-24