|Table of Contents|

Study on in vitro Culture and Regeneration System of Pepper（Capsicum annuum L.）(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年05
Page:
100-103
Research Field:
Publishing date:

Info

Title:
Study on in vitro Culture and Regeneration System of Pepper（Capsicum annuum L.）
Author(s):
XU Jian-zhong1; 2; CHEN Jin-fang1; 2; YANG Yu-fang2; SONG Wei-guang2; TIAN Hong2
(1.Engineering Research Center of Natural Pigment in Hebei Province，Handan，Hebei 057250；2.Chenguang Biotech Group Co.Ltd.，Handan，Hebei 057250)
Keywords:
pepper; plant regeneration; in vitro culture; cotyledon with petiole
PACS:
-
DOI:
10.11937/bfyy.201505031
Abstract:
The effect of different explants，age of seedling，different basic mediums and combinations of hormones on plant regeneration were investigated by using the variety of pepper（Capsicum annuum L.）‘South Korea pepper’ as experiment material.The results showed that cotyledon with petiole taken from 14-day-old seedlings was much easier to induce regeneration than cotyledon and hypocotyl.The optimal medium for the differentiation of pepper buds was MB+4.5 mg/L 6-BA+0.5 mg/L IAA+4 mg/L AgNO3+200 mg/L Cef，the medium for the bud elongation was MS+3 mg/L 6-BA+0.3 mg/L IAA+2 mg/L GA3+4 mg/L AgNO3+200 mg/L Cef and the medium for rooting culture was 1/2MS+0.4 mg/L NAA+4 mg/L AgNO3 and 1/2MS+0.4 mg/L NAA+0.2% activated carbon as subculture medium.

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Last Update: 2015-06-24