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Optimization of Primary Culture Conditions in vitro of Nandina domestica Thunb(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年05
Page:
93-96
Research Field:
Publishing date:

Info

Title:
Optimization of Primary Culture Conditions in vitro of Nandina domestica Thunb
Author(s):
LIU Zhong-bingMENG Yuan
(Department of Landscape Architecture,Wuhan Bioengineering Institute,Wuhan,Hubei 430415)
Keywords:
Nandina domestica Thunbin vitroprimary culturethe orthogonal experimental designthe percentage of induction
PACS:
-
DOI:
10.11937/bfyy.201505029
Abstract:
The regenerations of Nandina domestica was investigated by using in vitro stem sections by the orthogonal experimental design.Stem sections were cultured on different basic medium supplemented different concentrations of benzyladenine (BA) in combination with different concentrations of α-naphthaleneacetic acid (NAA) and sugar,the experiment was to select the optimum combiration,to improve callus formation.The results showed significant differences in the percentage of callus formation were observed among the basic medium,sugar,BA and NAA.The highest percentage of callus formation (74.4%) was obtained on MS medium with 1.0 mg/L BA,0.2 mg/L NAA and 20 g/L sugar.

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Last Update: 2015-06-24