[1]邓秀新,胡春根.园艺植物生物技术[M].北京:高等教育出版社,2005:162-164. [2]盛小光,顾宏辉,赵振卿,等.植物原生质体全能性表达及其在甘蓝类蔬菜育种上的应用[J].中国蔬菜,2011(16):1-8. [3]侯喜林,曹寿椿,佘建明,等.不结球白菜OguCMS下胚轴原生质体培养再生植株[J].园艺学报,2000,27(6):449-451. [4]李浚明,朱登云.植物组织培养教程[M].3版.北京:中国农业大学出版社,2005:178-186. [5]张金鹏,杨晶,韩玉珠,等.植物体细胞杂交的研究进展[J].北方园艺,2014(1):192-195. [6]武云霞,张鲁刚,张华敏,等.橙色大白菜原生质体的游离和纯化[J].北方园艺,2009(10):16-12. [7]邓红梅,马超,王颖.黄瓜幼苗子叶原生质体的分离纯化及融合条件优化研究[J].北方园艺,2013(11):6-9. [8]王卉,宁慧霞,刘敏,等.莴苣原生质体的分离方法[J].北方园艺,2012(11):109-112. [9]Huang H Y,Wang Z Y,Cheng J T,et al.An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system[J].Scientia Horticulturae,2013,150:206-212. [10]杨茹,刘世琦,张自坤,等.大蒜原生质体游离和纯化的研究[J].中国农学通报,2010,26(4):195-199. [11]黄玲.结球甘蓝原生质体培养及植株再生[D].金华:浙江师范大学,2011. [12]Yamashita H,Araki H,Yakuwa T.Plant regeneration from mesophyll protoptoplasts of Brussels sprouts(Brassica oleracea var. gemmifera Z.)[J].J Fac Agr Hokkaido University,1991,65(1):119-125. [13]卫志明,许智宏.花椰菜叶肉原生质体培养再生植株[J].园艺学报,1992,19(1):47-51. [14]Veera R N C,Gregory D N,Philip J D,et al.Regeneration from leaf explants and protoplasts of Brassica oleracea var.botrytis(cauliflower)[J].Scientia Horticulturae,2009,119:330-334. [15]戴永娟,和兆荣,胡靖锋,等.青花菜非对称体细胞杂交研究[J].湖南农业科学,2012(5):9-12,16. [16]侯喜林,曹寿椿,佘建明,等.不结球白菜子叶原生质体培养再生植株[J].南京农业大学学报,2000,23(4):17-20. [17]赵军良,逯保德,梁爱华,等.大白菜原生质体培养再生体系的优化[J].西北植物学报,2005,25(3):546-551. [18]叶志彪,李汉霞.红菜薹原生质体培养植株再生[J].园艺学报,1993,20(4):405-406. [19]Chen L P,Zhang M F,Hirata Y,et al.Efficient plant regeneration from cotyledon-derived protoplasts of cytoplasmic male-sterile tuber mustard (Brassica juncea Coss.var.tumida Tsen et Lee)[J].Acta Phytophysiologica Sinica,2001,27(5):437-440. [20]雷建军,陈世儒,郭余龙.叶用芥菜叶片原生质体再生植株[J].园艺学报,1992,19(1):52-56. [21]程振东,卫志明,许智宏.甘蓝型油菜下胚轴原生质体培养的研究[J].生物工程学报,1994,10(1):30-33. [22]Grzebelus E,Szklarczyk M,Baranski R.An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot[J].Plant Cell Tiss Organ Cult,2012,109:101-109. [23]宛新杉,王辅德,夏镇澳.芹菜原生质体培养条件与再生植株的研究[J].植物生理学通讯,1988(5):41-44. [24]韩清霞,沈火林,张振贤.芹菜胚性细胞悬浮系原生质体分离及再生植株[J].园艺学报,2007,34(3):665-670. [25]李宝平,张江涛,陈柔如,等.芫荽原生质体再生植株[J].植物学报,1991,33(12):932-937. [26]张冰玉,刘庆昌,翟红,等.甘薯及其近缘野生种种间体细胞杂种植株的有效再生[J].中国农业科学,1999,32(6):23-27. [27]卫志明,许智宏.蚕豆未成熟子叶原生质体再生植株[J].植物生理学通讯,1993,29(1):17-19. [28]李学宝,许智宏,卫志明,等.豇豆原生质体培养中体细胞胚胎发生和植株再生[J].植物学报,1993,35(8):632-636. [29]严成其,边自雄,张冬生,等.刀豆(Canavallia ensiformis)叶肉原生质体培养与植株再生[J].复旦学报(自然科学版),1991,30(4):381-387. [30]祁新,赵颖君,王艳秋,等.马铃薯悬浮细胞原生质体培养及植株再生[J].吉林农业大学学报,2000,22(1):52-55. [31]Iwamoto Y,Ezura H.Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives[J].Plant Biotechnology,2006(23):525-529. [32]张长远,吴定华.番茄及其近缘野生种茎段原生质体培养的研究[J].园艺学报,2000,27(3):216-217. [33]何晓明,王鸣,王之.辣椒子叶原生质体培养和植株再生[J].园艺学报,1997,24(3):298-300. [34]郭德章,鄢铮,赖钟雄,等.‘翠秀’黄瓜子叶原生质体的高效培养及植株再生[J].园艺学报,2003,30(2):227-228. [35]孙勇如,李向辉,孙宝林,等.新疆甜瓜子叶原生质体的培养和植株再生[J].植物学报,1989,32(12):916-922. [36]李贤,夏镇澳.茼蒿叶肉原生质体培养再生植株[J].植物生理学通讯,1990(1):27-20. [37]Chaipakdee W.Isolation and culture of protoplast from leaves of Lactuca sativa[J].Songklanakarin Journal of Science and Technology,2007,29(4):929-935. [38]Chen G Y,Conner A J,Christey M C,et al.Culture and regeneration of protoplasts from shoots of Asparagus cultures[J].International Journal of Plant Sciences,1997,158(5):543-551. [39]郭英华.生姜原生质体培养与体细胞变异[D].北京:中国农业大学,2005. [40]许修宏,孟琦,刘华晶,等.黑木耳菌丝原生质体的制备、再生及单核鉴定研究[J].东北农业大学学报,2011,42(8):96-100. [41]孙露,姚方杰,方明.毛木耳原生质体制备与再生条件的研究[J].中国食用菌,2012,31(3):35-37. [42]谢宝贵,朱虎.银耳原生质体分离与再生条件优化研究[J].菌物系统,2003,22(4):574-578. [43]王波,唐利民,熊鹰,等.双孢蘑菇原生质体分离与再生初探[J].西南农业学报,2004,17(2):215-216. [44]Li L H,Yin Q Y,Liu X H,et al.An efficient protoplast isolation and regeneration system in Coprinus comatus[J].African Journal of Microbiology Research,2010,4(6):459-465. [45]焦轶男,朱宏.菠菜原生质体游离与纯化的研究[J].生物技术世界,2012(12):1-3.
[46]Hegazi,Hegazi H,Sachiko M.Callus formation and plant regeneration from protoplast derived from Cotyledons and Hypocotyls of radish (Raphanus sativus L.) and other Cruciferous plants [J].Journal of the Japanese Society for Horticultural Science,1992,61(1)
:63-68. [47]杨光梅,杜春蕾,黄晓华,等.辣根叶片原生质体分离条件的研究[J].种子,2010,29(2):48-51. [48]陈劲枫,刘佩瑛,聂志平.菜豆叶肉原生质体培养[J].西南农业学报,1989(2):63-66. [49]吴耀武,马彩萍.豌豆叶肉原生质体的分离和培养及其愈伤组织的形成[J].西北植物学报,1990,20(4):269-274. [50]张兴国,刘佩瑛.冬瓜南瓜和西瓜原生质体培养(简报)[J].西南农业大学学报,1992,14(1):81-82. [51]李敬仁,孙勇如.西瓜子叶原生质体再生愈伤组织的获得[J].新疆农业科学,1992(5):218-219. [52]张兴国,刘佩瑛.丝瓜子叶原生质体培养研究[J].西南农业大学学报,1992,14(3):257-259. [53]苟小平,唐琳,赵军,等.苦瓜叶肉原生质体的培养[J].四川大学学报(自然科学版),1997,34(6):857-859. [54]张兴国,陈劲枫,张盛林,等.魔芋原生质体游离和培养条件研究[J].西南农业大学学报,1992,14(1):42-44. [55]陈丽,梁毅,汪李平,等.洋葱愈伤原生质体分离和纯化研究[J].中国农学通报,2011,27(31):147-151. [56]杨茹.大蒜原生质体融合与培养技术研究[D].泰安:山东农业大学,2010. [57]郭振.细胞生物学实验[M].合肥:中国科学技术大学出版社,2012:94-96. [58]Liu F,Ryschka U,Marthe F,et al.Culture and fusion of pollen protoplasts of Brassica oleracea L.var.italic with haploid mesophyll protoplasts of B.rapa L.ssp. Pekinensis[J].Protoplasma,2007,23:89-97. [59]张兰英,李耿光,陈如珠,等.菜心下胚轴原生质体培养和植株再生[J].植物学报,1994,36(2):105-110. [60]李贵,李必元,王五宏,等.结球甘蓝下胚轴原生质体培养再生植株体系的优化研究[J].西北植物学报,2012,32(12):2438-2443. [61]武恒.微流控芯片内植物原生质体的培养及其化学融合[D].杨凌:西北农林科技大学,2010. [62]蔡兴奎,柳俊,谢从华.马铃薯栽培种与野生种叶肉细胞融合及体细胞杂种鉴定[J].园艺学报,2004,31(5):623-626. [63]韩启厚,古瑜,于海龙.菜豆组织培养及遗传转化研究进展[J].中国农学通报,2013,29(10):97-101. [64]Geerts P,Druart P,Ochatt S,et al.Protoplast fusion technology for somatic hybridisation in Phaseolus[J].Biotechnologie,Agronomie,Société et Environnement,2008,12(1):41-46. [65]刘继红,徐小勇,邓秀新.原生质体再生植株变异及其在植物育种上的应用[J].华中农业大学学报,2003,22(3):301-306. [66]张延红.马铃薯原生质体再生植株遗传变异鉴定及RAPD稳定性研究[D].兰州:甘肃农业大学,2003. [67]李贵.结球甘蓝的离体再生以及体细胞无性系变异的RAPD分析[D].南京:南京农业大学,2012. [68]Lian Y J,Lin G Z,Zhao X M.Morphological,cytological,and molecular characterization of hybrids and their progenies derived from the somatic hybridization of Brassica campestris and Brassica oleracea[J].Chin J Biotech,2011,27(11):1586-1597. [69]Lian Y J.Production and characterization of a somatic hybrid of Chinese cabbage and cabbage[J].Chin J Biotech,2012,28(9):1080-1092. [70]Fock I,Collonnier C,Purwito A,et al.Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja[J].Plant Science,2000,160:165-176. [71]Fock I,Collonnier C,Jacques Luisetti,et al.Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato[J].Plant Physiol Biochem,2001,39:899-908. [72]司怀军,张宁,王蒂,等.利用体细胞杂交获取马铃薯软腐病的抗性[J].中国马铃薯,2006,20(4):193-197. [73]Tarwacka J,Polkowska-Kowalczyk L,Kolano B,et al.Interspecific somatic hybrids Solanum villosum(+) S.tuberosum,resistant to Phytophthora infestans[J].Journal of Plant Physiology,2013,170:1541-1548. [74]Collonnier C,Mulya K,Fock I,et al.Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L[J].Plant Science,2001,160:301-313. [75]连勇,刘富中,冯东昕,等.应用原生质体融合技术获得茄子种间体细胞杂种[J].园艺学报,2004,31(1):39-42. [76]Iwamoto Y,Hirai M,Ohmido N,et al.Fertile somatic hybrids between Solanum integrifolium and S.sanitwongsei(syn.S.kurzii ) as candidates for bacterial wilt-resistant rootstock of eggplant[J].Plant Biotechnology,2007(24):179-184. [77]Collonnier C,Fock I,Daunay M C,et al.Somatic hybrids between Solanum melongena and S.sisymbrifolium,as a useful source of resistance against bacterial and fungal wilts[J].Plant Science,2003,164:849-861. [78]王桂香,严红,曾兴莹,等.花椰菜—黑芥体细胞杂交获得抗黑腐病异附加系新材料[J].园艺学报,2011,38(10):1901-1910. [79]胡琼,李云昌.体细胞杂交在油菜细胞质雄性不育创建和改良中的应用[J].作物学报,2006,32(1):138-143. [80]谭芳,沈火林,王帅,等.芹菜与CMS胡萝卜原生质体非对称性融合的初步研究[J].园艺学报,2009,36(8):1169-1176. [81]司家钢,朱德蔚,杜永臣,等.原生质体非对称融合获得胡萝卜(Daucus carota L.)种内胞质杂种[J].园艺学报,2002,29(2):128-132. [82]Cappelle C,Morchen M,Hilbert J L,et al.Regeneration and molecular characterization of a male sterile interspecific somatic hybrid between Cichorium intybus and C.endivia[J].Plant Science,2007,172:596-603. [83]刘文明,李立,任丽,等.微流控细胞芯片生命分析应用多元化[J].分析化学,2012,40(1):24-31.