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Analysis for Genetic Diversity of Pleurotus ostreatus Hybrids(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
149-151
Research Field:
Publishing date:

Info

Title:
Analysis for Genetic Diversity of Pleurotus ostreatus Hybrids
Author(s):
SHENG Chun-ge; WANG Yan-feng; PAN Chun-lei; XU De-hai; HOU Guo-qiang
(Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences，Mudanjiang，Heilongjiang 157041)
Keywords:
Pleurotus ostreatus; hybrid generation; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.201504036
Abstract:
White strain (P1) and dark grey strain (P2) of Pleurotus ostreatus were used as dikaryon parents for single-spore crossbreeding.40 inbred lines P1×P2 were constructed，fruit-body color of 40 combines were observed.40 offspring and 2 parents were identified for genetic diversity by ISSR technique.The results showed that，6 ISSR primers generated 45 fragments，polymorphic bands was 34,polymorphism ratio 75.56%,the effective number of alleles (Ne)=1.4858，Nei’s gene diversity (H)=0.2725，Shannon information index (I)=0.4021，UPGMA based clustering separated the 42 test strains into 4 groups at an overall similarity coefficient of 0.6889 which had no directly contacts with its fruit-body color.The results showed that，a high level of genetic variation in P.ostreatus inbred lines.It would be beneficial to use various target varieties to broaden the genetic background of P.ostreatus.

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Last Update: 2015-04-21