|Table of Contents|

Study on Pathogen Identification and Biological Characteristics of Pathogenic Fungal of Poplar Leaf Margin Blight(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
119-123
Research Field:
Publishing date:

Info

Title:
Study on Pathogen Identification and Biological Characteristics of Pathogenic Fungal of Poplar Leaf Margin Blight
Author(s):
GAO Guo-ping; WANG Hong; DENG Qiu-yue; WANG Yue; XIE Wan-yu; MA Teng-fei
(School of Forestry，Shenyang Agricultural University，Shenyang,Liaoning 110161)
Keywords:
Populus; identification; biological characteristics; ITS rDNA
PACS:
-
DOI:
10.11937/bfyy.201504028
Abstract:
Taking the leaves infected by poplar isolated pathogenic bacteria as experimental materials，pathogenic bacteria was identified by morphology and molecular biology method，the effects of different culture medium,temperature,light,pH value,carbon source and nitrogen source on colony growth were studied by method of mycelium culture.The results showed that pathogen P1 was identified as Macrophoma sp..The optimum temperature of the mycelium growth was 25-30℃,the optimum pH value was 6,PA medium and PDA medium were suitable,and different illumination had no obvious effect on mycelium growth.The mycelium grew best on media with Lactose as carbon source，yeast as nitrogen resource.

References:

[1]曾大鹏.我国杨树病害的研究现状与防治[J].中国森林病虫，2002，21(1)：20-26. [2]徐素琴，原树忠，陈晓青.杨叶枯病菌：细链格孢(Alternaria tenuis)的研究[J].东北林学院学报，1984，12(1)：56-64. [3]项存悌.林病研究法[M].哈尔滨：东北林业大学出版社，1991. [4]方中达.植病研究方法[M].北京：中国农业出版社，1998. [5]喻璋，张猛.半知菌分属图册[M].北京：科学出版社,2009. [6]邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京：中国林业出版社,1984. [7]袁嗣令,周仲铭,邵力平.中国乔、灌木病害[M].北京：科学出版社,1997.  [8]陈捷.现代植物病理学研究方法[M].北京：中国农业出版社,2007. [9]刘小勇，田素忠，秦国夫,等.提取植物和微生物DNA 的SDS-CTAB改进法[J].北京林业大学学报，1997,19(13)：100-103. [10]刘晓倩，祁建民，陈玉森,等.中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析[J].中国农业科学，2012，45(17)：3515-3521. [11]Kumar S，Tamura K.Nei M1 MEGA3：Integrated software formolecular evolutionary genetics analysis and sequence alignment[J].Briefings in Bioinformatics，2004，5(2)：502-631. [12]马原松，裴冬丽，王文静，等.河南省几种白粉病的ITS序列分析[J].河南农业科学，2012，41(9)：87-90. [13]Ji H F，Yang Q，Song R H.Biological characters and rDNA ITS sequences of pathogen of poplar leaf blight[J].Journal of Forestry Research，2006，17(1)：17-20. [14]Kurtzman C P，Robnett C J.Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences[J].Antonie van Leeuwenhoek,1998，73(4)：331-371. [15]高国平，信雯，郑亚楠，等.刺槐心材腐朽菌的鉴定及其生物学特性[J].东北林业大学学报，2010，38(8)：60-62. [16]王丽丽，高国平，张伟，等.裂褶菌生物学特性的研究[J].辽宁林业科技，2009(6)：15-17.

Memo

Memo:
-
Last Update: 2015-04-21