|Table of Contents|

Molecular Cloning and Expression Analysis on PyDFR Gene of Pingguoli in Yanbian(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
99-103
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Expression Analysis on PyDFR Gene of Pingguoli in Yanbian
Author(s):
YANG Hong-xia; QU Bai-hong; LIU Zhen-kun; LU Yan-xin
(College of Agronomy,Yanbian University,Yanji，Jilin 133002)
Keywords:
Pingguoli; Dihyciroflavonol 4-reductase; gene cloning; sequence analysis; expression patterns
PACS:
-
DOI:
10.11937/bfyy.201504023
Abstract:
Taking the Pingguoli in Yanbian as material，the relationship between the expression and the accumulation of anthocyanin during the pigmentation of Pingguoli was analyzed by the homology cloning technology,and the real-time fluorescent quantitative PCR technique.The results showed that,the full-length cDNA of PyDFR included 1 039 basepair and opened reading frame encompassed 843 bp encoding a polypeptide of 281 amino acids residues with a calculated molecular weight(MV) of 32 kDa and isoelectric point theory(pI) of 6.32.The homology analysis revealed that this cDNA and Pyrus pyrifolia （JQ749637.1) was 99%.Real-time fluorescent quantitative RT-PCR results indicated the expression level of PyDFR were markedly enhanced in the sunlit side，and reached a maximum value at 1 day after debagging，then started decline rapidly，but finally expressed stably in the same level.The color code and anthocyanins content produced results essentially in agreement with these figures.In addition that had a certain role in promoting the accumulation of fruit anthocyanin.

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Last Update: 2015-04-20