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Rapid Propagation in vitro of Lonicera praeflorens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
95-98
Research Field:
Publishing date:

Info

Title:
Rapid Propagation in vitro of Lonicera praeflorens
Author(s):
WANG Huan1; 2; JIANG Zhe1; XU Shi-zhao1; LIU Yang1; YUAN Yu1
（1.Forestry College,Beihua University，Jilin,Jilin 132013；2.Jilin Province Key Laboratory of Forestry and Ecological Environment，Jilin,Jilin 132013）
Keywords:
Lonicera praeflorens Batalin; tissue culture; early spring plant
PACS:
-
DOI:
10.11937/bfyy.201504022
Abstract:
Taking tender stem section of Lonicera praeflorens which flowers in early spring in the Northeast as material，MS medium was selected as the basic medium supplemented with different plant growth regulators，the efficient process for the regeneration was studied.The results showed that the surface sterilization in 2.0% sodium hypochlorite for 20 minutes was the best.The optimum medium for the differentiation of lateral buds was MS+1.0 mg/L 6-BA+0.05 mg/L NAA+3.0% sucrose+0.7% agar；the medium MS+2.0 mg/L 6-BA+0.05 mg/L NAA+3.0% sucrose+0.7% agar was good for multiplication and the proliferation coefficient was 8.The optimum medium for inducing root was 1/2MS+0.3 mg/L IBA+0.3 mg/L NAA+1.5% sucrose+0.7% agar，and the rooting rate was 100%.

References:

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Last Update: 2015-04-20