|Table of Contents|

Changes of Free IAA and Relationship Between PsARP in Tree Peony(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年03
Page:
92-95
Research Field:
Publishing date:

Info

Title:
Changes of Free IAA and Relationship Between PsARP in Tree Peony
Author(s):
DING Xiao-wen1; CHENG Jie1; WU Yu-chuan2; ZHOU Rong-quan3; LAO Jun-feng1; MU Ping1
(1.College of Agronomy and Plant Protection，Qingdao Agricultural University，Innovation Team of Drought Resistance in Shandong Province，Qingdao,Shandong 266109；2.Jining Bureau of Agriculture，Jining,Shandong 272100；3.Rangu Agrotechnical Station，Dingtao,Shandong 274100)
Keywords:
Paeonia suffroticosa; dormancy release; free IAA; real-time PCR
PACS:
-
DOI:
10.11937/bfyy.201503028
Abstract:
Using Paeonia suffroticosa‘Luhehong’as plant materials，the expression pattern of PsARP and changes of free IAA were conducted under different chilling treatments.In order to reveal the changes of free IAA and the relationship between PsARP during dormancy release by chilling treatment in tree peony.The results showed that during the first 12 days of chilling treatment，the content of free IAA continuously reduced with the increase of chilling treatment.The lowest point was in accordance with the dormancy release of tree peony.In the later part of chilling treatment，although a reduction situation was also maintained，a relatively high level of content of free IAA was detected.Chilling treatment between 12 days and 18 days was a key point.By Real-time PCR，it showed that the expression of PsARP was enhanced and continuously increased in the buds of tree peony during the first step of dormancy release by chilling treatment，reaching the highest value during 12 days chilling treatment.At the second part of chilling treatment，the expression level of PsARP reduced to 50% than that of the highest level.The variation of free IAA could reflect the dormancy release degree of the flower bud.

References:

[1]刘波,郑国生,闫志佩,等.低温处理对牡丹春节催花及营养类物质变化的影响[J］.西北植物学报,2004,24(9):1635-1639.

[2]郑国生,盖树鹏,盖伟玲.低温解除牡丹芽休眠进程中内源激素的变化[J］.林业科学,2009,45(2):48-52.
3]Lang G A，Early J D，Martin G C.Endo-，para-and eco-dormancy：physiological terminology and classification for dormancy research [J］.Hort Science，1987，22:371-377.
[4]黄鑫.牡丹花芽内休眠解除相关基因的分离与功能分析[D］.北京:北京林业大学,2008.
[5]宋运贤,周素英,杜雪玲,等.低温预处理对小麦花药内源激素的影响[J］.核农学报,2012,26(7):1064-1069.
[6]陈小鹏，王秀峰，孙小镭，等.高效液相色谱法测定黄瓜瓜条中赤霉素和脱落酸含量[J］.山东农业科学，2005(1):65-67.
[7]吴耕西，毕桂红.高效液相色谱法测定苹果叶片中的吲哚乙酸和脱落酸[J］.山东农业大学学报，1994，25(1):51-55.
[8]钱利生，沈生荣，潘根生.茶树新梢内源激素的HPLC分析及日变化[J］.茶叶科学，1996，16(2):135-139.
[9]Nagar P K，Sood S.Changes in endogenous auxins during winter dormancy in tea (Camellia sinensis L.) O.Kuntze [J］.Acta Physiologiae Plantarum 2006，28(2):165-169.
[10]杨晓婉,郑国琦,许兴,等.宁夏枸杞果实生长发育期内源激素变化及关系研究[J］.西北植物学报，2013，33(1):116-122.
[11]Normanly J.Auxin metabolism [J］.Physiol Plant，1997，100：431-442.
[12]Ramaih S，Guedira M，Paulsen G M.Relationship of indole acetic acid and tryptophane to dormancy and preharvest sprouting of wheat [J］.Funct Plant Biol，2003，30:939-945.
[13]金小英，达世禄.赤霉素、3-吲哚乙酸和脱落酸的反相高效液相色谱测定法[J］．江西农业大学学报，1990，12(4):95-98.
[14]符继红，褚金芳，王吉德，等.固相萃取反相高效液相色谱荧光检测法测定拟南芥中的生长素[J］.分析化学，2009(9):1324-1327.
[15]王明月.高效液相色谱法测定发酵液中的吲哚乙酸含量[J］.热带农业工程，2009，33(5):1-3.
[16]马振宇，吕志华，姜廷福，等.高效液相色谱法测定菌肥中赤霉素和吲哚乙酸含量方法研究[J］.分析测试技术与仪器，2005，11(3):174-177.
[17]付蕾,王明林.苹果幼树梢尖3-吲哚乙酸、赤霉酸和脱落酸的高效液相色谱测定法的研究[J］.山东农业大学学报，1999，30(4):445-447.[18]侯凯,陈郡雯,申浩,等.川白芷内源激素的提取纯化和高效液相色谱法同步测定[J］.核农学报,2013,27(5):653-657.
[19]王彦杰,董丽,张超,等.牡丹实时定量PCR分析中内参基因的选择[J］.农业生物技术学报,2012，20(5)：521-528.
[20]徐丽华,刘春雷，常玉梅，等.双标准曲线相对定量PCR试验原理与方法[J］.生物技术通报，2011(1):70-75.
[21]Tillberg E.Levels of Endogenous indole-3-acetic acid in achenes of Rosa rugosa during dormancy release and germination [J］.Plant Physiology,1984，76:84-87.
[22]Falvre-Rampant O，Cardle L，Marshall D，et al.Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene [J］.J Experi Bot,2004，55(397)：603-612.
[23]陈伟,苏新国,郜海艳,等.低温对桃果实采后冷害及其内源激素的影响[J］.核农学报,2013,27(8):1173-1178.

Memo

Memo:
-
Last Update: 2015-03-03