|Table of Contents|

Optimization of SRAP-PCR System on Elytrigia repens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年02
Page:
91-97
Research Field:
Publishing date:

Info

Title:
Optimization of SRAP-PCR System on Elytrigia repens
Author(s):
MAO Wei; LI Pei-ying; SUN Zong-jiu
(College of Pratacultural and Environmental Science，Xinjiang Agricultural University，Key Laboratory of Grassland Resource and Ecology of Xinjiang，Urumqi，Xinjiang 830052)
Keywords:
Elytrigia repens; SRAP; system optimization
PACS:
-
DOI:
10.11937/bfyy.201502026
Abstract:
With genetic DNA of the Elytrigia repens as template，single factor and orthogonal design were applied to optimize the concentration of Mg2+,primer,dNTPs,DNA，Taq DNA polymerase for SRAP-PCR system of Elytrigia repens，the effect of different annealing temperature on reaction was studied by comprehensive analysis，the optimal system of SRAP-PCR was established.The results showed that the optimal system was Mg2+ 1.75 mmol/L，primer 0.15 μmol/L，dNTPs 0.20 mmol/L,DNA 50 ng/20μL，Taq DNA polymerase 0.75 U，2 μL 10 ×PCR in 20μL，concentrations of Mg2+ and primer had the highest effect on reaction，DNA concentrations had the least effect.The test of optimal system on 32 kinds of Elytrigia repens were done，the result was steady and clear，the establishing of optimal system made the base for the studies including genetic diversity and resistance marker and so on.

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Last Update: 2015-02-24