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Study on the Callus Induction and Multiplication of Rhododendron siderophyllum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年02
Page:
88-90
Research Field:
Publishing date:

Info

Title:
Study on the Callus Induction and Multiplication of Rhododendron siderophyllum
Author(s):
ZHOU Min1; LONG Xiu-qin2; LIU Yan3
(1.Zunyi Medical and Pharmaceutical College,Zunyi,Guizhou 563000；2.Guizhou Research Center of Karst Resource Environment and Development,Guiyang，Guizhou 550001；3.Guizhou Institutes of Biology,Guiyang，Guizhou 550009)
Keywords:
Rhododendron siderophyllum; callus; induction; multiplication
PACS:
-
DOI:
10.11937/bfyy.201502025
Abstract:
Taking cotyledon and hypocotyl of seed of Rhododendron siderophyllum as experiment material，the several key factors on callus induction and multiplication of Rhododendron siderophyllum in tussue culture were studied.The results showed that MS medium was suitable for induction and multiplication of callus.The concentrations of TDZ and NAA had a strong influence on callus induction.The MS medium supplemented with TDZ 0.50 mg/L and NAA 0.05 mg/L had high induction effect，and the induction rate was 77.78%.The MS medium supplemented with TDZ 0.10 mg/L and NAA 0.05 mg/L was suitable for callus multiplication，the multiplication rate could reach 96.67%，the multiplication ratio was 4.50.In order to keep callus multiplication more and more，the callus needed to transfer medium again and again.The ideal time for transferring medium was 40 days，so that it could avoid browning of callus.

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Last Update: 2015-02-24