|Table of Contents|

Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年01
Page:
145-147
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents
Author(s):
ZHOU Ying 1YUAN Meng-juan 1HAN Jun 1DONG Hui-jun 1ZANG Xiang-yin 2CHEN Fang 1
(1.College of Pharmacy,Liaocheng University,Liaocheng,Shandong 252059;2.Shandong Dong’e E Jiao Co.,Ltd.,Liaocheng,Shandong 252200)
Keywords:
Keywords: S.miltiorrhiza Bungeroot rotbiocontrol agentsidentification
PACS:
-
DOI:
10.11937/bfyy.201501039
Abstract:
Abstract:Taking rhizosphene soil of Chinese medicine S.miltiorrhiza Bunge root that from langkou town Chinese herbal medicine planting base in Chiping Connty Liaocheng city as material,used gradient dilution method and dual culture method,better inhibitory biocontrol agents to root rot were selected.The results showed that in the rhizosphere soil of Salvia miltiorrhiza Bunge discovered 10 strains beneficial microorganisms.And through the determination of reduplicative screening and its antibacterial spectrum,isolated 2 bacteriostatic strains:2-1 and 9-2.With the morphology and molecular biology identification,2-1 and 9-2 were bacillus subtilis and bacillus amyloliquefaciens.This lad a foundation for effective bio-control bacteria agent research and development.

References:

[1] 卫生部药典委员会,中国药典(一部)[M].北京:化学工业出版社, 〖LL〗1995:63. [2] Du G H,Zhang J T.The general situation and progress of the modern 〖JP5〗research of red sage root ( Radix salviae miltiorrhizae)[J].Herald of Medicine, 2004,23:355-360. [3] 文家富,陈光华,王刚云,等.丹参根部病害发生与综合防治技术[J].中国植保导刊,2009,29(10):32-33. [4] 王刚云,文家富,郑小惠,等.陕西商洛丹参根部病害调查研究[J].陕西农业科学,2009,55(3):53-54. [5] 朱伟杰,王楠,郁雪平,等.生防菌 Pseudomonas fluorescens 2P24对甜瓜根围土壤微生物的影响[J].中国农业科学,2010,43(7):1389-1396. [6] 刘晓妹,刘文波,范秀利.芒果细菌性黑斑病生防菌的筛选及防效测定[J].中国生物防治,2006(10):94-97. [7] 李旭,马福海,王秀娟,等.大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素[J].生态学杂志,2012,31(6):1453-1460. [8] 汪雪静.草莓尖孢镰刀菌根腐病生防细菌的分离鉴定及抑菌作用的初步研究[D].北京:北京农学院,2012:14-16. [9] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:267-388. [10] 中国科学院微生物所细菌分类组.一般细菌常用鉴定方法[M].北京:科学出版社,1978. [11] 陈开森,余阳,孙爱娣,等.一株马脲片球菌的分离、鉴定及其部分16SrDNA基因序列测定[J].江西医学院学报,2009,49(2):41-43. [12] 刘海龙,李春杰,许艳丽.生防细菌的抑菌谱和对大豆根腐病的防治[J].大豆通报,2008(1):10-13.

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Last Update: 2015-02-16