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Study on Rapid Proliferation of Stem Segments in vitro of Blueberry(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年01
Page:
103-105
Research Field:
Publishing date:

Info

Title:
Study on Rapid Proliferation of Stem Segments in vitro of Blueberry
Author(s):
ZHAO Xing-yu1; LI Li-li 2; 3; YANG Hong-yi 1
(1.College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040；2.Institute of Forestry Science of Heilongjiang Province,Harbin,Heilongjiang 150081；3.Institute of Crop Tillage and Cultivation,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)
Keywords:
Keywords:blueberry; explants; tissue culture; adventitious root
PACS:
-
DOI:
10.11937/bfyy.201501028
Abstract:
Abstract:Taking the wild blueberry seedlings in Greater hinggan mountains area as test materials,using the young stem segments as explants,the blueberry somaclone of rapid propagation system was explored.The results showed the explants disinfection treatment combination was 75% ethanol for 10-15 seconds and 0.1% mercuric chloride for 10 min.The medium was WPM,and the hormones were ZT 2.0 mg/L+NAA 0.2 mg/mL in the steps of inducement of callus and proliferation of adventitious buds.Adventitious roots were induced with dipping method by 200 μg/mL IBA.The results showed that callus and adventitious buds could be formed from stem of blueberry by the medium and hormones,and the induced ratio of adventitious buds was higher than 80%.In addition,the rooting rate was more than 70%.

References:

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Last Update: 2015-02-16