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Study on Optimization of Proliferation and Root Induction Technique of Anoectochilus roxburghii by Orthogonal Design(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年01
Page:
96-98
Research Field:
Publishing date:

Info

Title:
Study on Optimization of Proliferation and Root Induction Technique of Anoectochilus roxburghii by Orthogonal Design
Author(s):
HUANG Min; LIANG Chun-hui; LI Xiu-ping; YE Jian-rong; JIE Man
(Department of Tropical Crops,Guangdong AgriculturalIndustrialBusiness Polytechnic College,Guangzhou,Guangdong 510507)
Keywords:
Keywords: Anoectochilus roxburghii ; proliferation; root induction; orthogonal design
PACS:
-
DOI:
10.11937/bfyy.201501026
Abstract:
Abstract:Taking the test-tube seedling of Anoectochilus roxburghii as material,the optimization of culture medium for adventitious buds proliferation and rooting of Anoectochilus roxburghii were studied by orthogonal design L9(3 4).The results showed that,the effect for proliferation culture was in the order of 6-BA＞sucrose＞mashed bananas＞NAA,the optimizing medium for proliferation culture was MS+2.5 mg/L 6-BA+0.25 mg/L NAA+75 g/L mashed bananas+ 28 g/L sucrose with the multiplication coefficient of 2.725.The effect of the factor for root induction as follow：the basic culture medium＞6-BA＞activated carbon＞IBA,the optimizing medium for rooting culture was 1/2MS+0.25 mg/L 6-BA+5 mg/L IBA+2 g/L activated carbon and the number of roots per plantlets of 2.405.

References:

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Memo

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Last Update: 2015-02-13