Study on Extraction Technology of Lectin from Hericium erinaceus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: DENG Zheng-dong¹; CHENG Ai-fang¹; ZHENG Ting-jin¹; 2; 1.School of Life Science and Technology，Wuhan Institute of Bioengineering，Wuhan，Hubei 430415；
2.Institute of Molecular Medicine，Huaqiao University，Quanzhou，Fujian 362021

Keywords:: Hericium erinaceus; lectin; extraction technology; agglutinating activity

Abstract:: Taking Hericium erinaceus as material，lectin was extracted with PBS extraction method.On the basis of single factor tests of pH values of the extracting solvents，solid-liquid ratio，extraction time，extraction temperature，orthogonal experiment was designed to optimize the extraction condition of Hericium erinaceus lectin(HEL).The results showed that the extracting solvents was PBS，the concentration of sodium chloride was 0.10 mol/L.The best extraction technology was solid-liquid ratio 1∶15 （g/mL），pH values of the extracting solvents 6.5，extraction temperature 4℃,extraction time 24 hours.Under these conditions，agglutinating activity of HEL reached 0.586 and extraction rate reached 1.35%.

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Last Update: 2014-12-24