Study on Extraction Process of Total Flavonoid in Ficus tikoua(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: YANG Shen-ming¹; WANG Zhen-ji¹; 2; YANG Hong-wei¹; DU Ling-hai¹; 1.College of Chemistry and Life Science，Chuxiong Normal University，Chuxiong，Yunnan 675000；
2.Yunnan Province Universities Key Laboratory of Applied Biology，Chuxiong，Yunnan 675000

Abstract:: Taking Ficus tikoua Bur.as material，the total flavonoids in Ficus ticoua was extracted by means of ethanol refluxing method.The extraction technology of total flavonoids from Ficus ticoua was optimized through single factor experiment and orthogonal experiment.With the extraction rate of total flavonoids in Ficus ticoua as the indicator，effect of the volume fraction of ethanol，solid-liquid ratio，extraction temperature，extraction time on the extraction rate were studied.The results showed that the optimum extraction process parameters were followed,the volume fraction of ethanol 65%，ratio of solid to liquid 1∶25 (g∶mL)，extraction time 120 minutes，and extraction temperature 80℃.The average extraction rate of total flavonoids in male and female trees were 1.167% and 1.147% respectively，the average recovery rate was 98.56% under such condition.RSD value was 0.84%.The method has the advantages of simple operation，high accuracy，good precision，could be used as the extraction and content of total flavonoids in Ficus tikoua determination.

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Last Update: 2014-12-24