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Study on the Artificial Mutagenesis Method for Cucumber Green Mottle Mosaic Virus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年24期
Page:
108-111
Research Field:
Publishing date:

Info

Title:
Study on the Artificial Mutagenesis Method for Cucumber Green Mottle Mosaic Virus
Author(s):
ZHONG Min; ZHAO Xu-sheng; HE Yi-kun; WANG Ya-nan; CAO Ke-qiang
College of Plant Protection，Hebei Agricultural University，Baoding,Hebei 071001
Keywords:
cucumber green mottle mosaic virus; artificial mutation; attenuated strain
PACS:
S 642.2
DOI:
-
Abstract:
The wild isolate of cucumber green mottle mosaic virus was mutated by three artificial methods,including heat treatment，nitrous acid treatment and combination treatment,the attenuation effect of the three methods were compared.The results showed that all of the three treatments could attenuate virus to product smaller local lesion on Chenopodium amaranticolor. The attenuated rate of heat treatment，nitrous acid treatment and combination treatment were 11.41%，12.87% and 14.20% respectively.Chenopodium amaranticolor inoculated by wild strain was used as positive control，the attenuated rate of which was 1.99%.It presumed that the attenuation effect of combination treatment was the best.The result was useful to obtain CGMMV attenuated strain rapidly and effectively and set a good base for developing attenuated vaccine and studying the function of pathogenic genes in the future.

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Last Update: 2014-12-24