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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年24期

Page:

99-103

Research Field:

Publishing date:

Info

Title:

Study on Transformation of Multi-visual Marker Gene in Onion

Author(s):

ZHANG Hong-wei; TAN Wu-ping; LIANG Yi

Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097

Keywords:

onion; genetic transformation; anthocyanin

PACS:

S 633.203.6

DOI:

-

Abstract:

Taking onion seeds as materials,using transgenic method,the vector pBAC9075 was transformed into the callus,and the effects to transgenic plants of anthocyanin synthesis transcription factor Bi and C1 were studied either. The result showed that,189 regenerated plants were obtained after the regeneration and glyphosate screening.PCR analysis demonstrated that 5 regenerated plants were successfully transformed.Only 7 of the 189 plants were survived after field glyphosate screening and 2 of them showed anthocyanin accumulation.The above results was to provided a basis for the visualization genetic marker anthocyanins on early screening of transgenic plants in onion.

References:

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[4]刘海燕,冯冬茹,刘兵,等.农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究[J].热带亚热带植物学报,2009(3)：218-222.

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Memo

Memo:

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Last Update: 2014-12-24