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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年24期

Page:

84-87

Research Field:

Publishing date:

Info

Title:

Construction and Primary Characterization of cDNA Library for Wild Astragalus membranaceus of Changbai Mountain

Author(s):

QIN Jia-ze; QUAN Xue-li; TIAN Hai-li; JU Hong-guang; WU Song-quan

Agricultural College,Yanbian University,Yanji,Jilin 133002

Keywords:

Changbai mountain; wild Astragalus membranaceus; cDNA library; gene; secondary metabolism

PACS:

S 567.9

DOI:

-

Abstract:

Taking the roots and leaves of A.membranaceus of Changbai mountain as materials,total RNA was extracted reference the TRIzoL instructions,and the full-length cDNA library was constructed by SMART technology，in order to provided adequate gene resources for revealing the regulatory mechanism of secondary metabolism of Astragalus membranaceus at the molecular level.The results showed that the titer of unamplified library was 4.8×105 cfu/mL,the positive recombination rate was nearly 99.1%,the length of inserted fragments was larger than 0.5 kb,and the average length was 1.54 kb.The storage capacity,recombination rate and full-length efficiency of the constructed library could meet the requirements of screening genes for secondary metabolism in A.membranaceus.

References:

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Memo

Memo:

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Last Update: 2014-12-24