Taking the chlamydospore of Agrocybe chaxingu by grinding and enzyme decomposition method,the effect of environment condition factor on chlamydospore germination of?Agrocybe chaxinguwas studied.The results showed that the optimum temperature for germination was 25℃,the optimum pH value was 7.5,light could significantly inhibite the germination of chlamydospore,and the culture medium composition had no significant effect on the germination rate of chlamydospore.