|Table of Contents|

Optimization of Protein Extraction Process and Scavenging Function of DPPH Radical From Rapeseed Meal(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年14期
Page:
136-139
Research Field:
Publishing date:

Info

Title:
Optimization of Protein Extraction Process and Scavenging Function of DPPH Radical From Rapeseed Meal
Author(s):
LUO Qun1; ZHANG Hong2
1.College of Education Science,Chengdu Normal College,Chengdu,Sichuan 611130;
2.College of Life Science,Sichuan Normal University,Chengdu,Sichuan 610066
Keywords:
rapeseed meal; soluble protein; optimization; free radical scavenging
PACS:
S 794.4
DOI:
-
Abstract:
Taking hot-pressed rapeseed meal as material,the optimum protein extraction process by alkali extraction and acid precipitation was studied.The results showed that the optimal conditions of extraction were follows:pH 11.5,temperature 55℃,ratio of material to liquid 1∶15 g/mL,ultrasonic power 80 W,extraction time 100 min,3 times of extraction.Under these conditions,the extraction yield of soluble protein from rapeseed meal was 28.21%,the rapeseed protein yield of end product was 23.70%.The albumin and globulins by isolation of rapeseed protein had powerful scavenging efficiency to DPPH radical,the clearance rate were 31.09% and 29.08% respectively.

References:

[l]薛照辉，尉万聪，严奉伟，等，菜籽肽抗氧化活性的研究[J].中国油脂,2006,31(8)：48-50．

[2] Zhang S B,Wang Z, Xu S Y.Antioxidant and antithrombotic activities of rapeseed peptides[J]. Joumal of the American Oil Chemists’ Society,2008,85(6)：521-527.
[3]钮淡星，黄凤洪，倪光远，等．菜籽粕的饲用现状和饲用改良技术发展趋势[J]．中国油脂,2009, 34(5)：4-7．
[4]于冰宾．生物化学实验指导[M]．北京：清华大学出版社,2010:206-210．
[5]郑集，陈钧辉，生物化学[M]．北京：高等教育出版社,2002:70．
[6]严梅荣，王丹丹，胡蓉，等．膜分离制取可溶性菜籽蛋白及其对DPPH自由基的清除作用[J]．食品科学,2009,30(18)：148-151.

Memo

Memo:
-
Last Update: 2014-08-07