|Table of Contents|

Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年14期
Page:
119-123
Research Field:
Publishing date:

Info

Title:
Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride
Author(s):
LU Hai-juWANG BoPAN Liu-junMENG You-boZHANG Xiao-yong
Department of Life Science and Technology,Honghe College,Mengzi,Yunnan 661199
Keywords:
Trichoderma atroviridebiocontrolsolid fermentationconidial production
PACS:
S 476
DOI:
-
Abstract:
Taking Trichoderma atroviride isolate P3.9 isolated from trunk phloem of loquat as material,optimal substrates and its concentrations of medium,carbon source,nitrogen source,phosphate,water,inoclation,temperature and culture time were studied according to the mass conidial production by simple factor and orthogonal experiments,in order to screen out the best solid fermetation for biocontrol strain P3.9 of?Trichoderma atroviride.The results showed that the proper medium was rice bran,carbon source was glucose or D-fructose,nitrogen source was ammonium phosphate monobasic or dodium nitrate,phosphate was disodium hydrogen phosphate,water content was 40%,inoculation count was 9% and culture time was 28℃.The orthogonal experiment indicated that optimal medium of the highest yields of condia consisted 10 g rice bran,0.5% glucose,0.5% D-fructose,0.5% ammonium phosphate monobasic,0.05% disodium hydrogen phosphate.In addition,water content was 40%,inoculation count was 5%,culture temperature and time was respective 28℃ and 9 d (12 h light and 12 h dark).By the optimal medium and culture conditions,the maximum conidial yield was 7.5×10.9 conidia/g dry material.This conclusion provided theoretical parameter for the industrial fermentation.

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Last Update: 2014-08-07