Optimization and Application of Fluorescent-AFLP Analysis System on Cherry(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年14期

Page:

115-118

Research Field:

Publishing date:

Info

Title:

Optimization and Application of Fluorescent-AFLP Analysis System on Cherry

Author(s):

TIAN Chang-ping¹; ZHANG Fu-xing¹; LIU Xiao-jing²; ZHANG Xu¹; SUN Qing-tian¹

1.Yantai Academy of Agricultural Sciences,Yantai,Shandong 265500;

2.Fushan Fruit and Tea Technology Station,Yantai,Shandong 265500

Keywords:

cherry; fluorescent-AFLP; optimization; application

PACS:

S 662.5

DOI:

-

Abstract:

Taking 12 sweet cherry cultivars and 4 Chinese cherry cultivars as materials for establishing DNA-AFLP system.Several key factors affecting DNA-AFLP analysis such as DNA extraction,enzymes restriction,adaptor-ligation,pre-amplification and selective amplification were detected.The results indicated that CTAB method could be used for extracting ideal DNA from leaf of sweet cherry;200 ng DNA was digested completely by EcoR I and Mse I for 4 h.ligation products were diluted to 10 times for pre-amplification template and pre-amplification products were diluted to 20 times for selective amplification-64 pairs of primers were screened,of which 8 pairs could amplify clear,stable and satisfactory results an average of each primer band was 40.6.This study established a set of AFLP marker system for cherry,providing a foundation for studying genetic diversity and important agronomic traits.

References:

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