|Table of Contents|

Analyses of Sequence Variation and Genetic Diversity on 18S rDNA Regions of Auricularia auricula-judae(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年14期
Page:
111-115
Research Field:
Publishing date:

Info

Title:
Analyses of Sequence Variation and Genetic Diversity on 18S rDNA Regions of Auricularia auricula-judae
Author(s):
GUI Ming-ying1ZHAO Chun-yan1WU Su-rui1XIONG Yong2GUO Xiang1
1.Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooporatives,Kunming,Yunnan 650221;
2.Key Laboratory of Ethnic Medicine Resource Chemistry/State Ethnic Affairs Commission and Ministry of Education,Yunnan University of Nationalities,Kunming,Yunnan 650500
Keywords:
Auricularia auricula-judaes18S rDNAgenetic diversitysecondary structure
PACS:
S 646
DOI:
-
Abstract:
Used PCR method to amplify 18S rDNA regions of Auricularia auricula-judaes and sequenced them,and then used Clustalx 2,MEGA 5.0 software to analyze sequenced results and predicte RNA secondary structure.These results revealed the genetic diversity of five Auricularia auricula-judaes form Northeast China in order to provide reference for cultivation introduction and new variety breeding.The results showed the length of amplification regions was 1 700 bp.After removed ends of the poor quality area,the length of 18S rDNA sequences was 1 695 bp.The contents of (G+C) were 48.02~48.32%.There were 25 variable sites and 10 parsimony-informative sites in the whole anaylzed sequence.The variable sites mainly concentrated in 88~107 bp.The phylogenetic tree (N-J) showed 5?Auricularia auricula-judaes?clustered into a branch,and??Auricularia auricula?gathered in another.The 18S rDNA regions homology of Auricularia was consistent with that of traditional taxonomy.Nucleotide sequence combined with secondary structure of 18SrDNA region may be provide some molecular structure information for the classification of?Auricularia auricula-judaes

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Last Update: 2014-08-07