Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年14期

Page:

107-110

Research Field:

Publishing date:

Info

Title:

Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo

Author(s):

HUANG Zuo-xi¹; ZHANG Yang¹; YAN Xiao-yu¹; XIAO Xiao-jun¹; LI Qiang²; WANG Zi-bing²

1.Key Laboratory of Colleges and Universities for Research and Utilization of Distinctive Agricultural Undertakings,Neijiang Normal University,Neijiang,Sichuan 641112;

2.Sichuan Xiantuo Bio-technology Co.Ltd.,Neijiang,Sichuan 641110

Keywords:

Dendrobium officinale Kimura et Migo; protocorm; proliferation system

PACS:

R 931.71

DOI:

-

Abstract:

Taking the protocorm of T4 population as trial material,the effect of mass diameter,inoculation density of protocorm,hormone combinations,subculture cycle on proliferation and growth of protocorm were researched for establishing a highly efficient proliferation system of protocorm of Dendrobium officinale Kimura et Migo.The results showed that the high community effect could be observed during protocorm proliferation culture,so the appropriate increase of mass diameter and inoculation density of protocorm was of benefit to protocorm proliferation.Experiment was done with different concentration combinations of KT added with NAA or 6-BA added with NAA,which showed that NAA 0.02 mg/L+KT 0.5 mg/L was the best hormone combinations for the proliferation and growth of protocorm.50 d was the suitable subculture cycle for protocorm proliferation culture of Dendrobium officinale Kimura et Migo.

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