PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年14期

Page:

104-106

Research Field:

Publishing date:

Info

Title:

PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources

Author(s):

ZHANG Wen-long¹; 2

1.Pharmacy Department,Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002;

2.Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006

Keywords:

Dendrobium; ISSR; PAGE

PACS:

R 931.71

DOI:

-

Abstract:

Taking 16 Dendrobium germplasm resources as experimental materials,using polyacrylamide gel eletrophoresis(PAGE) methods,effect of difference of low molecular weight bands on plant taxonomy for?Dendrobium??were studied by 6 ISSR primers screened.The results showed that the results of the experiment was basically similar to the classical morphological classification.But there were some difference between both methods.PAGE method had high polymorphism ratio and could be used to further reveal genetic diversity and relationship of?Dendrobium??at the molecular level.

References:

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[2]胡之壁．中药现代生物技术[M].北京：人民出版社，2009：97-100.
[3] Saghai-Maroof M A. Soliman K M, Jorgensen R A, et aL RibosomalDNA spacerlength polymorphisms in barley: Mendelian inheritance. chromo-somal location, and population dynamics [J]. Proceedings of the National Academy of Sciences,1984, 81(24): 8014-8018.
[4]赵树进，中药材生物多样性及核酸分析技术[M]．北京：科学出版社，2009：64-70．
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Memo

Memo:

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