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Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年14期
Page:
101-104
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber
Author(s):
WANG Chang-tong; CAO Gang-qiang; LIANG Cong-min; XIE Bing-xin; YAN Fei-xiang
Biological Engineering Department,Zhengzhou University,Zhengzhou,Henan 450001
Keywords:
cucumber; NBS; RGA; bioinformatics
PACS:
S 642.2
DOI:
-
Abstract:
The bioinformatics of cucumber NBS resistance gene analogs (RGA gene) was analyzed using bioinformatics software (NCBI,DNAMAN,nnPredict,Antheprot,SOSUI,CBS,SWISS-MODEL).The physical and chemical properties,structure and function of the cucumber NBS resistance gene analogs (RGA gene) protein were predicted,in order to provide a theoretical basis for the further study on the regulation mechanism of disease resistance of cucumber.The results showed that the RGA gene encoded a length of 84 amino acid protein.Its molecular weight was 10 964.32 Da.The theoretical isoelectric point was 9.36.This gene had no transmembrane domain.It mainly was located in the cytoplasm.Evolutionary analysis indicated that the cucumber NBS resistance genes had the close genetic relationship with watermelon,also loofah.The secondary structure on this gene was α-β type,with the more obvious three helices and two fold.The protein had three functional sites:N-myristoylation site,Casein kianse II phosphorylation site and Protein kinase C phosphorylation site.And predicted that RGA protein could acts as a coenzyme role in the regulation of biochemical reactions,mediated regulation of gene expression in the stress resistant associated signaling pathways,as well as it could play an important physiological function during the development of seed embryos.

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Last Update: 2014-08-07