Study on the Identification of the Agrobacterium tumefaciens (Smith et Towns) Conn.and the Gene Interaction With the Transgenic 741 Populus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年14期

Page:

85-88

Research Field:

Publishing date:

Info

Title:

Study on the Identification of the Agrobacterium tumefaciens (Smith et Towns) Conn.and the Gene Interaction With the Transgenic 741 Populus

Author(s):

CAO Xiu-li¹; 2; MA Wan-lu¹; SUN Xu-xia¹; DU Ke-jiu²

1.Langfang Polytechnic Institute,Langfang,Hebei 065000；

2.College of Forestry,Resources and Engineering Agricultural University of Hebei,Baoding,Hebei 071000

Keywords:

Agrobacterium tumefaciens (Smith et Towns) Conn.; biological identification; transgenic 741 Populus; gene flow

PACS:

Q 943.2

DOI:

-

Abstract:

Taking Agrobacterium tumorigenic isolated from the rhizosphere soil of transgenic 741 Populus long tumour and non-GMO popalus tomentosa as experimental materials,with the methods of sections,staining and inoculated respectively,the morphology and biology of two Agrobacterium tumorigenic were identified.The results showed that the morphological characters were accord with the character of the Agrobacterium tumefaciens.Tumorigenicity test of carrot and tomato seedlings was positive;The bacterium induced tumour of carrot and to moto,at the same time,this bacterium could facilitate the rootage and the cancerization of the carrots and the tomato seedlings.The gene flow phenomena could not be found between this bacterium and its host after it was reinoculated to the transgenic 741 Populus.

References:

[l] Sule S.Biotypes of Agrobacterium tumEfaciens in Hungary[J]. Bacteriol,1978．44：207-213.
[2] Perry K L,Kado C I.Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains of Agrobacterium tume-faci ens [J].Bacteri01,1982 ,151: 343-350.
[3] Lele V C,Durgapal J C,Agarwal D K,et al.Crown and root gall ofgrape(Vitis vinifera) in Andra Pradesh[J]. Current Science ,1978,47:280.
[4] De Cleene M,Deley J. The host range of crown gaII[J]. Bot Rev,1976,42：389-466.
[5]喻子牛，何绍江，农业微生物学实验技术[M].北京：中国农业出版社,1996：312-325．
[6]刘芬，赵韶星．革兰氏染色三步法[J].山西职工医学院学报.2002(1)：54．
[7]陈则清，李朝晖．改良荚膜染色法检查新形隐球菌[J]．上海医学栓验杂志,1997,12(3)：185．
[8]张美莲，邢晓波，郭建，等，介绍一种快速细菌鞭毛染色法[J].陕西医学检验,2000,15(2)：29-30．
[9]曹秀利，郜秀荣，张昆，等，转双价抗虫基因741杨根际致瘤农杆菌分离及生长曲线测定[J].河北林果研究,2004,19(2)：105-107.
[10]周仲铭．林木病理学[M]．北京：中国林业出版社,1989：203-208．

Memo

Memo:

-

Common functions