1.The Key Lab of Nonwood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,Changsha,Hunan 410004；2.The Landscape and Famous Scenery Management Committee of Dahong Mountain,Suizhou,Hubei 431521
Using RNAiso-mate+modified CTAB method for RNA extraction of Vernicia fordii flower bud and detect RNA and protein by agarose gel electrophoresis and nucleic acid protein detector respectively,the method of RNA extraction of Vernicia fordii flower bud were studied.The results showed that the RNA of high quality,clear strip,high brightness,good integrity and purity was extracted by the method of RNAiso-mate+modified CTAB.The ratio of A260 and A280 was 1.97 and 1.93 respectively,yield was 176.9 and 154.2 μg/mL respectively.Based on the extracted high quality RNA,the VfSOC1?gene fragment was successfully cloned by reverse transcription polymerase chain reaction (RT-PCR).The length of VfSOC1 gene fragment was 611 bp and encode 203 amino acids.Compared with SOC1genes of Ricinus communis,Popμlus trichocarpa,Glycine max, VfSOC1 share 85%,84% and 78% identity respectively.The phylogenetic analysis showed that the genetic relationship of VfSOC1 and MvSOC1 was closer.