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Comparative Analysis of Induction and Somatic Embryo genesis of Different Origin in Sorbus pohuashanesis Hedl.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年13期
Page:
102-105
Research Field:
Publishing date:

Info

Title:
Comparative Analysis of Induction and Somatic Embryo genesis of Different Origin in Sorbus pohuashanesis Hedl.
Author(s):
ZHANG Jian-ying1; XU Chuan-ling2; SHEN Hai-long3; YANG Ling3; LI Jing1; TIAN Xin-hua1
1.Forestry Research Institute of Heilongjiang Province，Harbin,Heilongjiang 150080；
2.Dunhua Forestry Bureau，Dunhua，Jilin 133700；
3.College of Forestry，Northeast Forestry University，Harbin,Heilongjiang 150040
Keywords:
Sorbus pohuashanesis; induction of callus; somatic embryogenesis; origin; plant growth regulators
PACS:
S 718.43
DOI:
-
Abstract:
Taking the mature and immature zygotic embryos of Sorbus pohuashanensis Hedl.as explants, the effect of areas,development time and combinations of plant growth regulators on the induction of somatic embryos and callus induction were studied.The results showed that mature zygotic embryos were used as explants，the effect of MS+2.0 mg/L NAA+1.5 mg/L 6-BA on callus induction and somatic embryogenesis was good.Different origin of somatic embryogenesis differences for Dunhua>Harbin>Wuying>Shanhe.In order to immature zygotic embryos as explants，MS+0.05 mg/L 2,4-D+1.0 mg/L 6-BA callus induction rate was high，somatic embryogenesis in MS+2,4-D 0.2 mg/L+1.0 mg/L 6-BA was the most suitable.Different origin of somatic embryogenesis differences for Yanji>Harbin>Yichun.

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Last Update: 2014-08-02