|Table of Contents|

Isolation and Identification of A Bacillus vallismortis CZ and Primary Testing Its Disease-suppressive and Growth-promoting Capacities(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年13期
Page:
88-92
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of A Bacillus vallismortis CZ and Primary Testing Its Disease-suppressive and Growth-promoting Capacities
Author(s):
LIN Ying1; SI Chun-can2; ZHAO Qing-song1; DU Dao-lin2; LI Ping-ping1; 3
1.Key Laboratory of Modern Agricultural Equipment and Technology，Ministry of Education and Jiangsu Province,Institute of Agricultural Engineering，Jiangsu University，Zhenjiang,Jiangsu 212013；
2.School of Environment，Jiangsu University,Zhenjiang,Jiangsu 212013；
3.College of Forest Resources and Environment，Nanjing Forestry University，Nanjing,Jiangsu 210037
Keywords:
vinegar waste; antagonistic bacterial; isolated; disease-suppressive; growth-promoting
PACS:
S 476
DOI:
-
Abstract:
In order to seek new biocontrol agents，taking the vinegar residue substrate as the test materials，bacteria isolated from the vinegar residue substrate with inhibitory activities toward R.Solani using the dilution plate method，their broad antifungal inhibitory activity were tested using the dual plate assay,combining with morphological characteristics,16S rDNA sequence and physiological and biochemical characteristics to identify and determine its taxonomic status，its antagonistic potential against disease and plant growth promotion under greenhouse were further evaluated.The results showed that strain CZ isolated from the vinegar residue substrate exhibited broad-spectrum antagonism towards the tested fungi，including Fusarium oxysporum f.sp.niveum，Fusarium oxysporum f.sp.Melonis，Fusarium oxysporum f.sp.cucumerinum，Gibberella fujikuroi (Sawada) Wollenw，Fusarium graminearum，Glomerella cingulate.According to the morphological，phylogenetic analysis of the 16S rDNA sequence and physiological characteristics，strain CZ was identified as Bacillus vallismortis.Strain CZ also was found to be effective in controlling damping-off disease caused by Rhizoctonia solani under greenhouse conditions，inoculated with strain CZ led the percent disease index reductions in 18.3%.Together all，strain CZ has great potential as a biocontrol agent.

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Last Update: 2014-08-02