|Table of Contents|

Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年13期
Page:
84-87
Research Field:
Publishing date:

Info

Title:
Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’
Author(s):
MA Ling-ling; WANG Peng; WANG Shu-an; ZHANG Zhen-yu; YANG Ru-tong; LI Ya; LI Lin-fang
Institute of Botany,Chinese Academy of Sciences，Nanjing,Jiangsu 210014
Keywords:
‘Yuqiu No.1’; axillary bud induction; hormones; browning
PACS:
S 687
DOI:
-
Abstract:
Taking ‘Yuqiu No.1’ as material,the effect of methods of sterilization，types of basic culture medium，plant growth regulators and sampling time on axillary bud induction was studied.The results showed that significant difference was caused by using different culture mediums in which MS medium induced the most axillarys.The best axillary buds induction profile were explants soaked in carbendazim turbid liquid for 60 min，alcohol sterilization 30 s，soaked in mercuric chloride 9 min.The most suitable medium to induce axillary buds from ‘Yuqiu No.1’ was MS+0.8 mg/L 6-BA+0.10 mg/L IBA.The optimal sampling time to establish ‘Yuqiu No.1’ tissue culture system was in April，the contamination rate and browning rate were low，bud induction rate was high，axillary buds germinated and grew fast.

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Last Update: 2014-08-02