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Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年12期
Page:
99-102
Research Field:
Publishing date:

Info

Title:
Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages
Author(s):
GUO Li-li; LI Jun; LI Jin-hang; HOU Xiao-gai; KONG Xiang-sheng
College of Agriculture,Henan University of Science and Technology,Henan,Luoyang 471003
Keywords:
Paeonia suffruticosa Andr.; CTAB-LiCl improved method; extraction of total RNA
PACS:
S 685. 11
DOI:
-
Abstract:
Taking Paeonia suffruticosa Andr.cv.‘Luoyanghong’ as test material,total RNA were extracted by CTAB-LiCl improved method from tree peony leaves at small bells stage,big bells stage,circular peach stage,flat peach stage,flaw stage,initial bloom stage and fading stage.RNA integrity was detected by agarose gel electrophoresis,RNA purity was detected by ultraviolet spectrophotometer.The results showed that after electrophoresis,the RNA extracted in seven stages all had three bands-28S,18S and 5S rRNA,the ratio of A260/A280 ranged between 1.8 and 2.0.The rRNA bands at big bells stage,circular peach stage,flat peach stage,flaw stage,and initial bloom stage were tidy and clear,and the lightness of 28S rRNA band was higher than 18S rRNA band.The above showed that CTAB-LiCl improved method was suitable for extraction of total RNA from tree peony leaves,and big bells stage,circular peach stage,flat peach stage,flaw stage and initial bloom stage were the optimal five stages for total RNA extraction.

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Last Update: 2014-08-01