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 Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年12期
Page:
94-99
Research Field:
Publishing date:

Info

Title:
 Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker
Author(s):
LIU Zhi-xi; LI Zhi-qiang; WEI Hai-lian; ZHAO Zhong-lei; HE De; LI Cui-xin
College of Life Science,Southwest Forestry University,Kunming,Yunnan 650224
Keywords:
oyster mushroom(Pleurotus); ISSR; phylogenetic
PACS:
S 646.1+4
DOI:
-
Abstract:
Taking 53 varieties of oyster mushroom as materials,phylogenetic analysis of oyster mushrooms from different places were carried out by ISSR marker in order to provide some molecular biology evidences for research to varieties breeding and genetic relationship of mushroom.The results showed that from 27 ISSR primers,14 primers whose polymorphisms were obviously screened out.189 bands as well as 189 polymorphic bands were obtained from all the 53 tested strains,indicating that their polymorphism was 100%.The coefficients of genetic similarity ranged from 0.44 to 0.99.The results of cluster analysis showed that all 53 strains were classified into eight groups at 0.62 levels and that the coefficients of genetic similarity for majority of strains were pretty low,indicating that there were significant genetic variation and rich genetic diversity between the tested strains at the DNA level.The result also showed that there’s no significant correlation between the tested materials’ clustering and their geographic origins.

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Last Update: 2014-08-01