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Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年12期
Page:
84-88
Research Field:
Publishing date:

Info

Title:
Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato
Author(s):
LI Mei-qin; PEI Hua-li; QIAO Ning; LIU Yong-guang; XUE Qi-qin; LV Jin-fu
Biological Engineering Research and Development Center,Weifang University of Science and Technology,Weifang,Shandong 262700
Keywords:
coat protein gene; tomato yellow leaf curl virus(TYLCV); tomato; genetic transformation
PACS:
S 641.2
DOI:
-
Abstract:
Taking ‘Tedaruiguang’ tomato as material,plant expression vector 3301-CP containing TYLCV-CP genes was transferred into tomato cotyledons using Agrobacterium mediated transformation method.13 phosphinothricin-resistant plants were constructed and were detected by PCR and Southern Blot detection.The results showed 10 transformed tomato plants were positive reaction among the 13 phosphinothricin-resistant plants,the TYLCV-CP genes had been transferred into the tomato genome,and the transformation efficiency was 76.9%.The resistance to TYLCV of transgenic tomato plants was significantly stronger than non-transgenic tomato plants.

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Last Update: 2014-08-01