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Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年11期
Page:
125-129
Research Field:
Publishing date:

Info

Title:
Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris
Author(s):
YANG Shen-ming1; 2; WANG Zhen-ji1; 2; SHEN Ji-sen1
1.Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000;
2.Yunnan Province Universities Key Laboratory of Applied Biology,Chuxiong,Yunnan 675000
Keywords:
Adiantum capillus-veneris; total flavonoids; microwave extraction; orthogonal experiment
PACS:
S 682. 35
DOI:
-
Abstract:
Taking Adiantum capillus-veneris as material,total flavonoid content was measured by using Al(NO3)3-NaNO2 colorimetric method,effect of different methods on extraction rate of total flavonoid was studied.Based on the single factor experiment,using L9（34）orthogonal experiment,with the total flavonoids extraction rate as the indicator,effect of ethanol concentration,solid-liquid ratio,extraction power,extraction time on the extraction rate was studied in order to optimize the extraction technology of total flavonoids from Adiantum capillus-veneris.The results showed that the extraction rate of total flavonoids was the highest by using microwave method and it was simple operation,high accuracy and reliable.The optimum extraction process parameters were as followed:the volume fraction of ethanol 60%,ratio of material to liquid 1∶40 g/mL,extraction power 1 000 W,and extraction time 30 s.The measured total flavonoids extraction rate was 4.817% under such condition,and the average recovery was 99.48%,RSD＝2.30‰(n=5).

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Last Update: 2014-08-05