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Study on the Determination of Cercis chinensis Pollen Viability and Its Storage Method(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年11期
Page:
116-119
Research Field:
Publishing date:

Info

Title:
Study on the Determination of Cercis chinensis Pollen Viability and Its Storage Method
Author(s):
LI Rong
Henan Xinji Landscape Company,Xinxiang,Henan 453003
Keywords:
Cercis chinensi Bunge; pollen; germination; viability; pollen storage
PACS:
Q 946. 83
DOI:
-
Abstract:
Taking fresh pollen of Cercis chinesis as material,pollen viability was tested by some methods including culture in vitro,I2-KI staining,carmine acetate dyeing and TTC,in order to study characteristics of pollen germination and the suitable method of pollen storage. The results showed that culture media with different concentration of sucrose and boric acid had good effect on the pollen germination.The pollen germination rate reached 8864% after cultured on the media supplemented with 15% sucrose,0.004% boric acid and 0.08% agar.I2-KI staining and TTC staining were not suitable for testing pollen viability of Cercis chinensis,carmine acetate dyeing could be used to test pollen viability of Cercis chinensis.Pollen vitality decreased as time gone on,4℃ dry storage was the best storage condition to keep the vitality of pollen.Pollen could be stored over 26 d with significant viability.

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Last Update: 2014-08-05