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cDNA PCR Library of mRNA From Dendrobium candidum Leaves Constructed Using RACE Technique(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年11期
Page:
85-88
Research Field:
Publishing date:

Info

Title:
cDNA PCR Library of mRNA From Dendrobium candidum Leaves Constructed Using RACE Technique
Author(s):
FENG Pei-chun; CUI Yi-hui; ZHANG Zi-feng; ZHU Qian-kun; WANG Wan-jun
School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031
Keywords:
cDNA PCR library; RACE; reverse transcription; PCR
PACS:
R 931. 71
DOI:
-
Abstract:
Based on RACE technique,total RNA from Dendrobium candidum leaves was subjected to reverse transcription and terminal deoxynucleotidyl transfer resulting the cDNA which contained the specific sequences in two ends.The obtained cDNA with the specific sequences was amplified using PCR with primers which matched the specific sequences to establish cDNA PCR library.The results showed that PCR amplification of the cDNA PCR library was more efficient significantly than that of cDNA.The quantity of cDNA was magnified more than one hundred times by the constructed cDNA PCR library.By the cDNA PCR library constructed using 5 pg total RNA as material,the satisfactory PCR amplification results can be obtained.This construction method of the cDNA PCR library was time and labor saving.This method would provided the technical basis for cloning genes of low expression.

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Last Update: 2014-08-05