|Table of Contents|

Study on Pathogen Identification and Biological Characterstics of the Leaf Spot of Calotropis gigantean (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年10期
Page:
112-116
Research Field:
Publishing date:

Info

Title:
Study on Pathogen Identification and Biological Characterstics of the Leaf Spot of Calotropis gigantean
Author(s):
XIE Mei-hua12LI Lin3LI Xue-ling12YANG Hai-yan12CHEN Hua-hong12WANG Zhen-ji12
1.Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000;
2.Yunnan Province Applied Biology Key Laboratory of University,Chuxiong,Yunnan 675000;
3.Information Center,Chuxiong Pharmaceutical College,Chuxiong,Yunnan 675000
Keywords:
Calotropis gigantealeaf spotpathogen identificationbiological characterstics
PACS:
S 59
DOI:
-
Abstract:
One fungal strains was separated from the leaf spot of Calotropis gigantean,noted for CX008.It was identified through the combination of morphological observation and modern molecular biological technique,the culture traits of it were studied based on the morphological characteristics.The results showed that:the pathogen is Alternaria tenuissima(Nees ex Fr.).The fungi was able to use 7 kinds of carbon and nitrogen sources.The best carbon and nitrogen source for its growth were glucose and beef extract respectively.The mycelial could grow at 15~40℃,the approprate temperature for its growth was 25~30℃,mycelial stopped growing at 5℃,10℃and 45℃.Its spore was able to germinate at 15~40℃,the approprate temperature was 28℃,it could not germinate when temperature exceed 40℃.12 h light and dark alternative was helpful to mycelial growth,photoperriod had little influence on spore germination.In laboratory test,there were inhibitory action for 5 kinds broad-spectrum fungicide on mycelial growth.Iprodione was the best getrmicides on the tested,then Metalaxyl mancozeb,Chlorothalonil and Mancozeb as follow,the worst was Fenaminosulf.

References:

[1]中国科学院华南植物研究所,海南植物志[M].3卷,北京:科学出版社,1974:255.
[2]中国科学院植物志编辑委员会,中国植物志[M].63卷,北京:科学出版社,1977:384-386.
[3]傅登祺,黄宏文.能源植物资源及其开发利用简况[J].武汉植物学研究,2006,24(2):183-190.
[4]李瑞,曾建立,王晓东,等,耐盐碱植物牛角瓜产能成分分析[J].过程工程学报,2007,7(6):1217-1220.
[5] Ahmed U A M, Bashier N H H, Shi Z H.Evaluation of insecticidal potentialities of extracts from Colotropis procera Ait.against Henosepilachna elaterii Rossi[J].江文学学报(农业与生命科学版),2006,32(3):292-299.
[6]魏静,赵元藩,张燕平.牛角瓜的组织培养[J].林业调查规划,2013,38(2):112-115.
[7]李克烈,罗联忠,陈伟,等.牛角瓜的栽培管理技术及应用前景[J].广西农业科学,2007,26(3):247-249.
[8]王茂媛,戴好富,王祝年.牛角瓜根脂溶性成分的GC-MS分析[J].热带作物学报,2010,31(11):2039-2042.
[9]高柱,王小玲,牛角瓜开发价值及栽培技术研究[J].北方园艺,2011(18):202-206.
[10]李璇,费魏鹤,李丑东,等.牛角瓜纤维/粘胶混纺产品定量分析方法[J].上海纺织科技,2013,41(4):13-21.
[11]方中达.植病研究方法[vrJ,北京:中国农业出版社,1998.
[12]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001.
[13]张中义,冷怀琼,张志铭,等.植物病原真菌学[M].成都:四川科学技术出版社,1988:172,374-376.
[14]巴尼特HL,亨特BR半知菌属图解[M].沈崇尧,译,北京:科学出版社,1977: 66-67, 78-79 .82-83, 130-131, 132-133, 152-153.
[15]张天宇,张敬泽,陈伟群,等,中国真菌志(第十六卷,链格孢属)[M].北京:科学出版社,2003.
[16]刘晓龙,三十种真菌病害检索表[J].中药材,1987(2):34.
[17]严吉明,叶华智,四川药用植物病害调查与病原鉴定Ⅱ.四川药用植物病害[J].西南农业学报,2008,21(2):359-363.
[18]童晓茹,王学翠,温学森,等.植物叶片真菌病害生物防治的研究进展[J].山东科学,2008,21(1):41-46.
[19]刘艳,叶建仁.植物病害潜伏慢染研究进展[J].南京林业大学学报,2002,24(5):69-72.
[20]刘纪凤,范运梁,植物叶片病害的发生与防治[J].植物保护,2008(20):115.
[21]马琼,秦恩华,李又萍,魔芋白绢病病原菌的分离鉴定[J].安徽农业科学,2006,33(12):2307,2320.
[22]赵国柱,张天宇,中国砖格丝孢菌研究I.砖格孢属[J].菌物系统,2003,22(1):19-22.
[23]高芬,吴元华,链格孢属(Alternaria)真菌病害的生物防治研究进展[J].植物保护,2008,34(3):1-6.
[24]严清平,袁善奎.5种链格孢属植物病原真菌对10种杀菌剂的敏感性比较[J].植物保护,2008,34(2):124-127.
[25] Von Arx J久Die Arten der Guttg ColLetrichum[J].Phytopahtol Z,1957,29:413-468.
[26]孙广宇,彭友良,李振歧,等,核苷酸序列分析在真菌系统学研究中的应用[J].西北农林科技大学学报(自然科学版),2003,31(6):187-192.

Memo

Memo:
-
Last Update: 2014-08-06