|Table of Contents|

Study on in vitro Culture of Actinidia kolomikta Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年10期
Page:
97-101
Research Field:
Publishing date:

Info

Title:
Study on in vitro Culture of Actinidia kolomikta Leaves
Author(s):
ZHANG Yu-jie1YANG Zhong2GU De-feng1
1.College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118;
2.Dehui City Agricultural Technology Education Center,Dehui,Jilin 130300
Keywords:
Actinidia kolomiktaleaveculture in vitro
PACS:
S 663.4
DOI:
-
Abstract:
Effect of different combinations of plant growth regulators on callus induction,differentiation and rooting of the test tube seedlings were studied by using leaves of Actinidia kolomikta as explants.The results showed that the optimal medium for callus inducement from leaf was MS+ZT 1.0 mg/L+NAA 0.2 mg/L,and callus inducement rate reached 91.67 percent;the best medium for differentiation of adventitious buds was MS+ZT 2.0 mg/L+IBA 0.2 mg/L,and the differentiation rate was 89.45 percent with an average number of 8.11 budding.Actinidia kolomikta was extremely easy to rooting,and to add different concentrations of IAA,IBA and NAA on 1/2MS medium,rooting rate were very high,and most even achieved 100 percent;as matrix consisted of garden soil,peat and sand by a volume ratio of 1∶1∶1 cultures the test tube seedlings,the survival rate was 92 percent.

References:

[1]朱有昌.东北药用植物[M].哈尔滨:黑龙江科学技术出版社,1989:740-741.
[2]张继有,严仲铠,长白山植物药志[M].长春:吉林人民出版社,1982:983.
[3]李平亚,赵春芳,刘金平.猕猴桃的化学成分和生物活性研究进展[M].长春:吉林科学技术出版社,2006.
[4]李结维,毛世忠,梁木源,等,猕猴桃属植物果实营养成分的研究[J].广西植物志,1995,15(4):377-382.
[5]常晓丽,马冰如,何玲,狗枣猕猴桃叶化学成分的研究(I)[J].中草药,1993 ,24(6):283-285.
[6]常晓丽,陈仑,马冰如.狗枣猕猴桃化学成分研究[J].中草药,1996,27(7):395.
[7]赵继红,梁宇,颜达予,黄酮类化合物抗氧化活性的结构因素[J].北方工业大学学报,2001,13 (1):36-42.
[8]吴怀恩,甄汉深,钟振国.猕猴桃属植物的研究进展[J].中药材,2004,27(1):59-63.
[9]欧阳红涛,华卡,董建辉,猕猴桃药理作用的研究进展[J].中国医药导报,2007,32(4):12-13.
[10] Hirsch A M The culture of Actinidia chinensis fruit tissues and themetabolism of free amino acids in Stem segments cultured锄vitro [J].Comptes Rendus Hebdomadaires des Seances de l’ Academic des Sciences D,1975,280(11):1369-1372.
[11]桂耀林,猕猴桃离体茎段愈伤组织的诱导和植株再生[J].植物生理学通讯,1979,21(4):339-343.
[12]朱俊义,夏广清,刘雪莲,等.东北刺人参组培快繁及种质保存技术[J].东北林业大学学报,2007,35(11):9-10.
[13]刘金英,徐有明,李双来,等.佛手山药组织培养的研究[J].植物研究,2006,26(3):323-237.
[14]李瑞芬,张敬原,赵茂林.结缕草愈伤组织诱导及植株再生[J].园艺学报,2003,30(3):355-357.
[15]张喜春,吴绛云.狗枣称猴桃叶片植株再生及无性系快速繁殖[J].北方园艺,1990(9):7-9.

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Last Update: 2014-08-06