|Table of Contents|

Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年09期
Page:
159-162
Research Field:
Publishing date:

Info

Title:
Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes
Author(s):
ZHOU Hui-ming1; ZHANG Yan-zhen1; XI Ya-li2; WEI Sheng-long2
1.Lincang Teachers’ College,Lincang,Yunnan 677000；
2.Mushroom Research Institute，Hexi University，Zhangye，Gansu 734000
Keywords:
Lyophyllum decastes; submerged culture; carbon source; nitrogen source; mycelia biomass; exopolysaccharid
PACS:
S 646.19
DOI:
-
Abstract:
Taking Lyophyllum decastes as material,the effects of different C and N-sources on mycelial biomass and exopolysaccharide production by five strains of Lyophyllum decastes were studied by single factor liquid fermentation experiment.The aim was to screen the optimum C-sources,N-sources and suitable fermenting strains for Lyophyllum decastes liquid culture,which was the basis of exploring its main factory fermentation.The results showed that the best C-source of strains ZY01,ZY02 and ZY03 was sucrose,the best N-source was peptone,followed by offals,urea.The mycelium biomass of strains ZY04 and ZY05 was most with glucose and peptone as optimum C and N-sources,sucrose was the best C-sources for its exopolysaccharide yield,the best N-sources was respectively urea,peptone.The mycelium biomass (12.626 g/L) and exopolysaccharide production (11.5148 mg/L) of strain ZY02 was most with combination of 2% sucrose+0.2% peptone and 2% sucrose+0.3%peptone,respectively.Therefore,the effect of different C and N-sources on mycelial biomass and exopolysaccharide production was not a positive correlation,strain ZY02 and ZY03 was respectively the suitable fermenting strains of two kinds of indicators.

References:

[1]戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1)：1-21.

[2]席亚丽,茆爱丽,王晓琴,等.荷叶离褶伞子实体、菌丝体及发酵液蛋白质营养价值评价[J].菌物学报,2010,29(4)：603-607.

[3]席亚丽,王治江,王晓琴,等.荷叶离褶伞子实体、菌丝体和发酵液营养成分比较分析[J].食品科学,2010,31(6)：155-157.

[4]Pokhrel C P,Ohga S.Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes[J].Food Chemistry,2007,105(2)：641-646.

[5]Ukawa Y,Ito H,Hisamatsu M.Antitumor effects of (1-->3)-beta-D-glucan and (1-->6)-beta-D-glucan purified from newly cultivated mushroom,Hatakeshimeji (Lyophyllum decastes Sing.)[J].Journal of Bioscience and Bioengineering,2000,90(1)：98-104.

[6]Ukawa Y,Furuichi Y,Kokean Y,et al.Effect of Hatakeshimeji(Lyophyllum decastesSing.) mushroom on serum lipid levels in rats[J].Journal of Nutritional Science and Vitaminology,2002,48(1)：73-76.

[7]Miura T,Kubo M,Itoh Y,et al.Antidiabetic activity of Lyophyllum decastesin genetically type 2 diabetic mice[J].Biological and Pharmaceutical Bulletin,2002,25(9)：1234-1237.

[8]NakamuraT,Itokawa Y,Tajima M,et al.Radioprotective effect of Lyophyllum decastes?and the effect on immunological functions in irradiated mice[J].Journal of Traditional Chinese Medicine,2007,27(1)：70-75

[9]张汉燚,王治江,席亚丽,等.荷叶离褶伞中试发酵条件与培养基优化研究[J].中国酿造,2012,31(1)：96-99.

[10]Ukawa Y,Izumi Y,Ohbuchi T,et al.Oral administration of the extract from?Hatakeshimeji(Lyophyllum decastesSing.) mushroom inhibits the development?of atopic dermatitis-like skin lesions in NC/Nga mice[J].Journal of Nutritional Science and Vitaminology,2007,53(3)：293-296.

[11]张汉燚,张芬琴,王小明,等.荷叶离褶伞子实体营养成分分析与评价[J].菌物学报,2008,27(5)：696-700.

[12]张春梅,宋海,魏生龙.荷叶离褶伞菌丝体多糖的提取及还原力的研究[J].中国食用菌,2012,31(6)：44-48.

[13]张凤琴,李彩霞,李鹏,等.荷叶离褶伞可溶性多糖提取工艺研究[J].食品工业科技,2010(5)：224-225.

[14]余海尤,曹春蕾,崔宝凯.忍冬木层孔菌液体培养过程中多酚含量及抗氧化活性研究[J].菌物学报,2012,31(6)：933-939.

[15]谢占玲,何智媛,唐龙清,等.五株羊肚菌碳、氮源及适合发酵菌株的筛选[J].食用菌学报,2009,16(1)：43-46.

[16]叶明,陈吴西,彭伟,等.分批与流加培养对萼状粒毛盘菌生物量和胞外多糖产量的影响[J].浙江大学学报(农业与生命科学版),2010,36(6)：630-634.

[17]ShihI L,Chou B W,Chen C C,et al.Study of mycelial growth and bioactive?polysaccharide production in batch and fed-batch culture of Grifola frondosa[J].Bioresource Technology,2008,99(4)：785-793.

[18]Vinarta S C,Molina O E,Figueroa L C,et al.A further insight into the practical applications of exopolysaccharides from Sclerotium rolfsii[J].Food Hydrocolloids,2006,20(5)：619-629.

[19]徐鹏,钱竹,董亮,等.灵芝深层发酵生产胞外多糖和灵芝酸的动力学分析[J].应用与环境生物学报,2008,14(4)：562-565.

[20]CuiJ,Chisti Y.Polysaccharides of Coriolus versicolor:physiological activity,use,and production[J].Biotechnology Advances,2003,21：109-122.

[21]苗玉志,张微帷,何兵.鲜块菌片热风干燥工艺参数的优化[J].现代食品科技,2013,29(1)：162-166.

[22]LeungM Y K,Liu C,Koon J C M,et al.Polysaccharide biological response?modifiers[J].Immunology Leters,2006,105：101-114.

[23]Wasser S P.Medicinal mushroom as a source of antitumor and immunomodulating polysaccharides[J].Appl Microbiol Biotechnol,2002,60：258-274.

[24]Cui J,Kim Tha Goh K,Archer R,et al.Characterisation and bioactivity of protein bound polysaccharides from submerged-culture fermentation of CoriolusversicolorWr-74 and ATCC-20545 strains[J].Microbiol Biotechnol,2007,34：393-402.

[25]HuangH C,Liu Y C.Enhancement of polysaccharide production by optimization?of culture conditions in shake flask submerged cultivation of Grifol aumbellata[J].Journal of the Chinese Institute of Chemical Engineers,2008,39(4)：307-311.


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Last Update: 2014-08-13