|Table of Contents|

Preliminary Study on Paulownia Transformation via Pollen-mediated Transformation Approach Based on GFP Observation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年09期
Page:
117-120
Research Field:
Publishing date:

Info

Title:
Preliminary Study on Paulownia Transformation via Pollen-mediated Transformation Approach Based on GFP Observation
Author(s):
YANG Li-yan1; RAN Hui1; QIAO Bai-lu1; SUN Yi2; 3
1.College of Life Science,Shanxi Normal University,Linfen,Shanxi 041004;
2.Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;
3.Key Laboratory of Crop Gene Resources and Germplasm Enhancementon Loess Plateau,Ministry of Agriculture,Taiyuan,Shanxi 030031
Keywords:
Paulownia; ultrasonication; pollen-mediated transformation; green fluorescence protein (GFP)
PACS:
S 792.43
DOI:
-
Abstract:
Taking Paulownia pollen as receptor,the fresh Paulownia pollen was treated under ultrasonication mixed with plasmid DNA harboring green fluorescence protein (GFP) gene,the expressions of GFP gene in pollen grains,pollen tubes were observed under a fluorescence microscope.The results showed that 100 g/L sucrose was the suitable concentration for in vitro pollen germination.Pollen grains had strong green autoflorescent background,so GFP gene was not a reliable reporter for tracing the transformation of exotic genes in pollen.Pollen tubes in the treatment group produced strong green florescence compared to the control which meant the GFPgene expression in pollen tubes.The present study preliminarily provided evidence for Paulownia genetic transformation via pollen-mediated transformation approach.

References:

[1]钟启宏,孔繁瑞,孙威,等.通过花粉管途径建立泡桐转化系统的初探[J].遗传,1994,16(6):16-19.

[2]施士争,倪善庆.泡桐组织培养系统性研究初探[J].江苏林业科技,1995,22(3):20-22.

[3]FanG Q,Zhai X Q,Zhai C J,et al.Callus induction from leaves of different?Paulownia?species and its plantlet regeneration[J].Journal of ForestryResearch,2001,12(4):209-214.

[4]范国强,彭海风,翟晓巧,等.泡桐叶片蛋白质多态性及其聚类分析[J].植物学通报,2001,18(6):739-774.

[5]范国强,翟晓巧,蒋建平,等.不同种泡桐叶片愈伤组织诱导及其植株再生[J].林业科学,2002,38(1):29-35.

[6]范国强,翟晓巧,马新业.两种泡桐叶片体细胞胚胎发生及植株再生[J].核农学报,2005,19(4):274-278.

[7]范国强,董占强,李峰稳,等.光周期对泡桐叶片离体植株再生影响研究[J].西北植物学报,2007,27(1):104-109.

[8]Kalaycioglu H,Deniz I,Hiziroglu S.Some of the properties of particleboard made from Panlownia[J].Journal of Wood Science,2005,51:410-414.

[9]刘飞,范国强,董占强.泡桐离体开花培养系统的建立[J].林业科学,2007,43(12):56-63.

[10]孙毅,王景雪,崔贵梅.超声波处理花粉介导植物基因转化方法[P].中国：ZL991211529，20060329.

[11]Wang J X,Sun Y,Cui G M,et al.Transgenic maize plants obtained by pollen-mediated transformation [J].Acta Botanica Sinica,2001,43：275-279.

[12]Wang W Q,Wang J X,Yang C Q,et al.Pollen-mediated transformation of Sorghum bicolor?plants [J].Biotechnology Applied Biochemistry,2007,48:79-83.

[13]Wang J X,Li Y H,Liang C.Recovery of transgenic plants by pollen mediated transformation in?Brassica juncea[J].Transgenic Research,2008,17:417-424.

[14]Eapen S.Pollen grains as a target for introduction of foreign genes into plants:an assessment [J].Physiology and Molecular Biology of Plants,2011,17:1-8.

[15]Sambrook J,Maniatis T,Fritsch E F.Molecular Cloning:A Laboratory Manual (2ndedition)[M].New York:Cold Spring Harbor Labrotory Press，1989.

[16]陈志远.泡桐属（Paulownia）花粉形态学的初步研究[J].武汉植物学研究,1983,1(2):143-147.

[17]ChenX,Equi R,Baxter H,et al.?A high-throughput transient gene expressionsystem for switchgrass (Panicum virgatumL.) seedlings [J].Biotechnologyfor Biofuels,2010,3:1-10.

[18]Ottenschlager I,Barinova I,Voronon V,et al.Green fluorescent protein (GFP) as a marker during pollen development [J].Transgenic Research,1999,4:279-294.



Memo

Memo:
-
Last Update: 2014-08-13