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Study on the Genetic Diversity of Walnut Germplasm Resources Based on RAMP Marker(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年09期
Page:
106-108
Research Field:
Publishing date:

Info

Title:
Study on the Genetic Diversity of Walnut Germplasm Resources Based on RAMP Marker
Author(s):
ZHANG Jian-ying1; 2; MAO Xiang-hong1; 2; ZHANG Ying-ying1; 2
1.Hebei Engineering and Technology Research Center of Improved Forest Variety,Shijiazhuang,Hebei 050061;
2.Hebei Academy of Forestry Science,Shijiazhuang,Hebei 050061
Keywords:
walnut; RAMP markers; germplasm resources; genetic diversity; cluster analysis
PACS:
S 664.1
DOI:
-
Abstract:
Taking walnut as material,34 walnut germplasm resources were analyzed by RAMP technology,in order to establish genomic DNA extraction method.The results showed that eleven primers amplificated 185 DNA bands totally,142 bands among which were polymorphic.The rate of polymorphic bands were 62.5%~93.3%.The dissimilarity coefficient among samples were 0.0274 to 0.4848,which indacted that 34 walnut germplasm had abundant genetic diversity.RAMP wass a feasible approach for DNA fingerprint of walnut germplasm resources.The genetic basis of walnut had a certain regional features,and ‘Shimen’ walnut was a relatively independent cultivated population in genetic relation.

References:

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Last Update: 2014-08-13