[1]Brown G R,Carlson J E.Molecular cytogenetics of the genes encoding 18S-58S-26S rRNA and 5S rRNA in two species of spruce (Picea)[J].Theor?Appl Genet,1997,95:1-9. [2]刁英.染色体核型研究的方法及应用[J].渝西学院学报,2004(3):55-58. [3]Langer?P R,Waldrop A A,Ward D C.Enzymatic synthesis of biotin-labeled?polynucleotides:novel nucleic acid affinity probes[J].Proc Natl Acad Sci USA,1981,78:6633-6637. [4]Wu Q,Liu F,Li S H,et al.Uniqueness of the Gossypium mustelinum?enome revealed by GISH and 45S rDNA FISH[J].Journal of Integrative Plant Biology,2013,55(7):654-662. [5]Pizzaia D，Oliveira V M，Martins A R，et al.Karyotype characterization reveals active 45S rDNA sites located on chromosome termini in Smilax rufescens (Smilacaceae)[J].Genet Mol Res,2013,12(2):1303-1310. [6]Németh?A V,Dudits D,Molnár-Láng M,et al.Molecular?cytogenetic characterisation of Salix viminalis?L.using repetitive DNA sequences[J].J Appl Genetics,2013,54:265-269. [7]Sultana S,Lee S H,Bang J W，et al.Physical mapping of rRNA gene loci and inter-specific relationships in wild Lilium?distributed in Korea[J].J Plant Biol,2013,53:433-443. [8]Dou Q W,Chen Z G,Liu Y A,et al.High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J].Breeding Science,2009,59:651-656. [9]Liu B，Qi L，Chen R.Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii[J].Biological Research,2007,40:23-28. [10]Liu B,Zhang S,Zhang Y,et al.Molecular cytogenetic analysis of four?Larix?species by bicolor fluorescence in situ hybridization and DAPI banding[J].International Journal of Plant Sciences,2006,167(2):367-372. [11]Kim J,Childs K L,Islam-Faridi M N,et al.Integrated karyotyping of sorghum by in situ hybridization of landed BACs[J].Genome,2002,45:402-412. [12]Dong F,Song J,Naess S K，et al.Development and applications of a set of chromsome-specific cytogenetic DNA markers in patato[J].Theor Appl Genet,2000,101:1001-1007. [13]Shibata F,Nagaki K,Yokota E,et al.Tobacco karyotyping by accurate centromere identification and novel repetitive DNA localization[J].Chromosome Res，2013,21:375-381. [14]Ried T，Baldini A，Rand T C,?et al.Simultaneous visualization of seven different DNA probes by in stiu?hybidization using combinatorial fluorescence and digital imaging microscopy [J]. Proc Natl Acad Sci USA,1992,89:1388-1392. [15]Nederlof P M，van der Flier S，Vrolijk J,et al.Fluorescence ratio measurements of double-labeled probes for multiple in situ?hybridization by digital imaging microscopy[J] . Cytometry, 1992，13（8）:839-845. [16]Levsky J M，Singer R H.Fluorescence in situ hybridization:past，present and future[J].Journal of Cell Science,2003,116:2833-2838. [17]Hasterok R,Langdon T,Taylor S,et al.Combinatorial labeling of DNA probes enables multicolour fluorescence in situ hybridization in plants[J].Folia?Histochemica Et Cytobiologica, 2002,40(3):319-323. [18]董凤平,韩素英,张守功,等.25S rDNA在杨属植物染色体上的定位[J].云南植物研究,2007,29(4):423-428. [19]Lengerova M,Kejnovsky E,Hobza R,et al.Multicolor FISH mapping of the dioecious model plant，Silene latifolia[J].Theor Appl Genet,2004,108:1193-1199. [20]Vischi M,Jurman I,Bianchi G，et al.Karyotype of Norway spruce by multicolor FISH[J].Theor Appl Genet,2003,107:591-597. [21]Schwarzacher T，Leitch A R，Bennett M K,et al.In situ?localization of parental genomes in a wide hybrid[J].Ann Bot,1989,98:315-324. [22]Neelam K，Rawat N，Tiwari V K,et al.Introgression of group 4 and 7 chromosomes of Ae.peregrinain wheat enhances grain iron and zinc density[J].Mol Breeding,2011,28:623-634. [23]Chester M,Gallagher J P，Symonds V V,et al.Extensive chromosomal variation in a recently formed natural allopolyploid species,Tragopogon miscellus (Asteraceae)[J]. PNAS, 2012, 6：1176-1181. [24]Maluszynska J,Hasterok R.Identification of individual chromosomes and parental genomes in Brassica juncea?using GISH and FISH[J].Cytogenetic and Genome Research, 2005, 109: 310-314. [25]Han F,Fedak G,Benabdelmouna A,et al.Characterization of six wheat×Thinopyrum intermedium?derivatives by GISH，RFLP and multicolor GISH[J].Genome,2003,46:490-495. [26]Han F，Liu B，Fedak G，et al.Genomic constitution and variation in five partial amphiploids of wheat-Thinopyrum intermedium?as revealed by GISH，multicolor GISH and seed storage protein analysis[J].Theor Appl Genet,2004,109:1070-1076. [27]Zoller?J F,Yang Y，Reinhold G,et al.Comparative genomic in situ hybridization?(cGISH) analysis on plant chromosomes revealed by labeled Arabidopsis?DNA[J].Chromosome Res,2001(9):357-375. [28]Pinkel D，Langegent J,Collins C,et al.Fluorescence in situ?hybfidization with human chromosome-specific libraries:Detection of trisomy 21 and translocations of chromosome 4[J].Proc Natl Acad Sci USA,1988,85:9138-9142. [29]Houben A，Field B L，Saunders V A.Microdissection and chromosome painting of plant B chromosomes[J].Methods in Cell Science,2001,23:115-124. [30]Yu F，Lei Y T,Li Y，et al.Cloning and characterization of chromosomal markers in alfalfa( Medicago sativa L.)[J].Theor Appl Genet,2013,126:1885-1896. [31]Lysak M A，Pecinka A,Schubert I.Recent progress in chromosome painting of Arabidopsis?and related species[J].Chromosome Res,2003(11):195-204. [32]Almeida?C,Pedrosa-Harand A.High macro-collinearity between?lima bean (Phaseolus lunatus?L.) and the common bean (P.vulgaris?L.) as revealed by comparative cytogenetic mapping[J].Theor Appl Genet,2013,126:1909-1916. [33]Budiman M A，Chang S,Lee S,et al.?Localization of jointless-2?gene in the centromeric region of tamato chromosome 12 based on high resolution genetic?and physical mapping[] . Theor Appl Genet,2004,108:190-196. [34]Islam-Faridi M N,Childs K L,Klein P E,et al.A molecular cytogenetic map of sorghum chromosome 1:fluorescence in situ?hybridization analysis with mapped bacterial artificial chromosomes[J].Genetics,2002,161:345-353. [35]Dong F,Song J,Naess S K,et al.Development and applications of a set of chromsome-specific cytogenetic DNA markers in patato[J].Theor Appl Genet,2000,101:1001-1007. [36]Zhang P，Li W，Friebe B,et al.Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH[J].Genome,2004,47:979-987. [37]Nakamura R,Kitamura S，Inoue M，et al.Karyotype analysis of Nicotiana kawakamii?Y.Ohashi using DAPI banding and rDNA FISH[J].Theor Appl Genet,2001,102:810-814. [38]LiuJ Y,She C W,Hu Z L，et al.A new chromosome fluorescence bandingtechnique combining DAPI staining with image analysis in plants[J].Chromosoma,2004,113:16-21. [39]Yurkevich O Y，Naumenko-Svetlova A A,Bolsheva N L,〖JP3〗et al. Investigation of genome polymorphism and seed coat anatomy of species of section Adenolinum from the genus Linum[J].Genet Resour Crop Evol,2013，60:661-676. [40]Urdampilleta J D,Coulleri J P，Ferrucci M S,et al.Karyotype evolutio n and phylog enetic analyses in the genus Cardiospermum L.(Paullinieae，Sapindaceae)[J].Plant Biology， ?2013(15):868-881. [41]Karlov G I,Danilova T V，Horlemann C,et al.Molecular cytogenetics in hop (Humulus lupulusL.) and identification of sex chromosomes by DAPI-banding[J].Euphytica,2003,132:185-190. [42]LiuJ Y,She C W,Hu Z L,et al.A?new chromosome fluorescence banding?technique combining DAPI staining with image analysis in plants[J].Chromosoma,2004,113:16-21.