|Table of Contents|

Study on Uniform Design Methods for Optimization of Plant Regeneration of Aquilaria sinensis (Lour.) Spreng. in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年08期
Page:
100-104
Research Field:
Publishing date:

Info

Title:
Study on Uniform Design Methods for Optimization of Plant Regeneration of Aquilaria sinensis (Lour.) Spreng. in vitro
Author(s):
JIA Xian12JIA Rui12WANG Ying2WU Kun-xin2CHEN Xiong-ting2
1.College of Agronomy,Hainan University,Haikou,Hainan 570228;
2.Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101
Keywords:
Aquilaria sinensis (Lour.) Spreng.tissue cultureuniform designoptimization
PACS:
S 772.3+7
DOI:
-
Abstract:
Taking the stems of Aquilaria sinensis (Lour.) Spreng. as explants,using DPS, the whole system for shoot regeneration were established through uniform design experiments.The results showed that the medium of 7/10 MS+6-BA 0.35 mg/L+NAA 0.01 mg/L was suitable for buds induction,and the rate of starting was 93.33%;the medium of 7/10 MS+KT 1.27 mg/L+NAA 0.2 mg/L was suitable for bud multiplying,and the multiplying coefficient was 3.53;the rooting medium which was 7/10 MS+6-BA 0.02 mg/L+IBA 1.23 mg/L+CM 25 mL/L was suitable with the rate of induction of 33.33%.The medium supplement for regeneration system of Aquilaria sinensis (Lour.) Spreng. was optimized,which provides a basis support for its propagation and construction of genetic transformation system.

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Last Update: 2014-08-12