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Effect of Different Hormone Combination on Adventitious Bud Differentiation of Strawberry Leaf(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年08期
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Effect of Different Hormone Combination on Adventitious Bud Differentiation of Strawberry Leaf
Author(s):
WANG Ting-tingCAI RuiWU HuiZHOU Xiao-fu
Key Laboratory of Plant Resources Science and Green Production of Jilin Province,College of Sciences and Technology,Jilin Normal University,Siping,Jilin 136000
Keywords:
strawberry leafTDZdark treatmentadventitious bud
PACS:
S 668.4
DOI:
-
Abstract:
Taking the leaves of strawberry ‘Jingyao’ as explants,the effect of different hormone combination,AgNO3 and dark culture on adventitious bud were investigated.The results showed that the optimum medium for adventitious bud regeneration was MS supplenmented with 1.0 mg/L thidiazuron(TDZ),0.4 mg/L indole-3-butyric acid(IBA) and 2.0 mg/LAgNO3,under this conditions,the isolated regeneration rate of adventitious bud from leaf explant reached 70.3%,and the average number of regenerated buds reached 4.2;AgNO3 could effectively restrain strawberry leaf vitrification and brown stain;dark treatment promoted adventitious bud differentiation of strawberry leafs,the best time of dark treatment was 14 d.

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Last Update: 2014-08-12