|Table of Contents|

Screening and Identification of Antagonistic Endophytic Actinomycetes Isolated from Sophora alopecuroides L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年08期
Page:
86-91
Research Field:
Publishing date:

Info

Title:
Screening and Identification of Antagonistic Endophytic Actinomycetes Isolated from Sophora alopecuroides L.
Author(s):
QI He-xing; HU Mei-juan; WANG Li; ZHOU Xing-chen; GU Pei-wen
School of Agriculture，Ningxia University，Yinchuan,Ningxia 750021
Keywords:
Sophora alopecuroides L.; endophytic actinomycetes; antimicrobial activity; identification; 16S rRNA sequence analysis
PACS:
S 476
DOI:
-
Abstract:
Taking six kinds of plant pathogenic fungi as target bacteria，culture dishes method was adopted,antibacterial activity of 126 endophytic actionmycetes strains isolated from .alopecuroides L.were tested.The results showed that 15 strains had strong inhibiting effect on target pathogens，accounting for 11.9% of total isolates.Of them，inhibition zones of YWZKDS4 against Pyricularia oryzae and NDZKDS65against Fusarium oxysporum reached 15.12 mm and 18.54 mm respectively,NDZKDS22 had broad-spectrum antifungal activity，their inhibition zones against 5 species of target pathogens were more than 10 mm.The closest genetic relationships of YWZKDS4 with Streptomyces scabiei,NDZKDS22with S.albospinus，and NDZKDS65with S.capillispiralis were found respectively by morphological characteristics and 16S rRNA sequence similarity analysis.Sequence similarity were 99.1%，96.4%，98.8%.According to the experiment，the antimicrobial activity of endophytic actinomycetes from ?S.alopecuroides L.against 6 species of target plant pathogens was better.

References:

[1]Stone J K ， Bacon C W ， White J F.Microbial Endophytes[M].New York:Marcel Dekker ， 2000:3-29.

[2]王莉衡.植物内生菌的研究进展[J].化学与生物工程，2011,28(3):5-7.

[3]Schrey S D，Tarkka M T.Friends and foes:streptomycetes as modulators of plant disease and symbiosis[J].Antonie Van Leeuwenhoek，2008，94(1):11-19.

[4]龙澍普,赵久顺,于荣.宁夏苦豆子的价值与资源利用[J].内蒙古农业科技,2012(4):127-128.

[5]江苏新医学院.中药大辞典[M].上海:上海科技出版社,1986:1293.

[6]顾沛雯,郝丽,徐润,等.宁夏灵武白芨滩国家级自然保护区苦豆子内生放线菌区系分析[J].宁夏大学学报（自然科学版）,2011,32(4):380-385.

[7]郝丽,胡美娟,马海龙,等.宁夏干旱荒漠区苦豆子内生放线菌的分离鉴定[J].农业科学研究.2011,32(2):18-21.

[8]周德庆.微生物实验手册[M].上海:上海科学技术出版社,1997.

[9]胡美娟,周星辰,王丽,等.苦豆子内生放线菌产酶特性分析及活性菌株鉴定[J].北方园艺,2013(16)：98-102.

[10]姜淑梅,张龙,戴世鲲,等.一种简单,有效的适用于PCR操作的放线菌DNA提取方法[J].生物技术,2007(1):39-41.

[11]Lee S B,Tayler J W.Isolation of DNA from fungal mycelia and single spores[M].A Guide to Methods and Application,1990.

[12]顾沛雯.宁夏干旱荒漠区苦豆子内生颉颃细菌的筛选及鉴定[J].粮食安全与植保科技创新,2009:676-680.

[13]顾沛雯.宁夏干旱荒漠区苦豆子内生颉颃细菌的筛选及其抑菌物质研究初报[J].中国植保导刊,2009,29(1):5-8.

[14]顾沛雯.苦豆子内生放线菌的分离鉴定及其拮抗菌筛选[J].北方园艺,2009(6):12-16.

[15]郁蕾,戴好富,曾艳波,等.白刺链霉菌15-4-2中的抗MRSA活性成分研究[J].中国抗生素杂志,2012，37(2):94-97.

[16]曾会才,曾涛,陈汉清,等.一种防治香蕉枯萎病的白刺链霉菌株BWL15-4及其应用[P].中国：CN2011102960933，2012125.

[17]姚领爱,胡之璧,王莉莉,等.植物内生菌与宿主关系研究进展[J].生态环境学报,2010,19(7):1750-1754.



Memo

Memo:
-
Last Update: 2014-08-12