|Table of Contents|

Effect of Different Nitrogen Sources on Dendrobium candicum Wall.ex Lindl Suspension Cultures(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年07期
Page:
152-155
Research Field:
Publishing date:

Info

Title:
Effect of Different Nitrogen Sources on Dendrobium candicum Wall.ex Lindl Suspension Cultures
Author(s):
LIAN Hua-shan1; 2; YANG Gui-xian2; 3; LI Huan-xiu2
1.Chengdu Vocational College of Agricultural Science and Technology,Chengdu,Sichuan 610000；
2.College of Horticulture，Sichuan Agricultural University,Ya’an,Sichuan 625014；
3.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030
Keywords:
Dendrobium candicum; nitrogen; proliferation; polysaccharides; protein
PACS:
R 931.71
DOI:
-
Abstract:
Taking Dendrobium candicum as material,with 1/2 MS as the basic medium,20 mmol/L different types of additional nitrogen sources were used,the effect of different nitrogen sources on Dendrobium candicum suspension culture proliferation,polysaccharides content and protein content were studied.The results showed that most conducive Dendrobium candicum suspension culture proliferation was glycine,followed by nitrate,and the worst was glutamate.In the accumulation of polysaccharide,the most obvious of polysaccharides accumulate was Ca(NO3)2.On the contrary,lysine was not conducive to polysaccharides accumulation for Dendrobium candicum.KNO3 and (NH4)2SO4 were conducive Dendrobium candicum accumulation of protein,urea and other nitrogen were not conducive to the accumulation of the protein for Dendrobium candicum.Comprehensive analysis found that Ca(NO3)2 was the most conducive for Dendrobium candicum suspension culture.

References:

[1]冉懋熊.名贵中药材绿色栽培技术[M].北京：科学技术文献出版社,2002.

[2]吴志刚,刘贤旺,张寿文.石斛属植物组织培养研究进展[J].中药研究与信息,2005(1)：23-25.

[3]胡忠,吉星和.黑节草种苗的大量培养[J].植物杂志,1979(3)：1.

[4]何静波,李勇.黑节草原胚体的繁殖[J].云南植物研究,1982,4(2)：211.

[5]张治国,刘骅,王黎.铁皮石斛原球茎增殖的培养条件研究[J].中草药,1992,23(8)：431.

[6]郭顺星,曹文岑,张集慧.铁皮石斛人工种子制作流程及发芽研究[J].中草药,1996,27(2)：105.

[7]徐红,李晓波,王铮涛,等.海南、广东省石斛属植物资源与种质保护研究[J].中国野生植物资源，1999(5)：24-27.

[8]秦廷豪.铁皮石斛的组织培养与快速繁殖[J].热带农业科学,2008,28(1)：25-29.

[9]李进进.铁皮石斛茎段离体初代培养研究[J].作物杂志,2010(1)：79-80.

[10]何涛,淳泽,汪天杰,等.铁皮石斛腋芽的快速繁殖[J].中国野生植物资源,2010,29(1)：58-61.

[11]蒋向辉,佘朝文,王善粉.不同激素浓度对铁皮石斛高效快繁体系的影响[J].江苏农业科学,2009(6)：76-78.

[12]汤亚飞,蔡时可,郑锦荣.铁皮石斛组织培养与快速繁殖(简报)[J].亚热带植物科学,2010,39(2)：74-75.

[13]Xu H,Lu J,Wang Z T,et al.The scavenging of reactive oxygen species by crude drugs and cultured tissues of five species of?Dendrohium?Sw[J].J Plant Resour Environ,2011,10(2)：35-37.

[14]Gao J,Jin R,Wu Y,et al.Comparative study of tissue cultured?Dendrobium protocorm on immunologrcal function [J].J Chin Med Mater,2002,25(7)：487-489.

[15]洪森荣,肖波,江静.KT和NAA对铁皮石斛带芽茎段生长发育的影响[J].江苏农业科学,2012,40(1)：55-56.

[16]陈希,黄丹丹.铁皮石斛的组织培养与快速繁殖研究进展[J].海峡药学,2011,23(8)：16-23.

[17]苏江,罗兴录,何铁光,等.铁皮石斛原球茎固体培养和液体悬浮培养动力学研究[J].西北农业学报，2007,16(4)：161-165.

[18]宋经元,郭顺星,肖培根.铁皮石斛原球茎液体悬浮培养的研究[J].中草药,2004,35(9)：1042-1046.

[19]Hahlbrock K,Scheel D,Logemann E,et al.Oligopeptide elicitor-mediated defense gene activation in cultured parsley cells[J].Proc Natl Acad Sci USA,1995,92(10)：4150-4157.

[20]魏小勇.铁皮石斛原球茎悬浮培养研究[J].现代中药研究与实践,2004,18(4)：7-11.

[21]韩晓红,段春红,阎贺静,等.铁皮石斛原球茎液体悬浮培养研究进展[J].安徽农业科学,2010,38(20)：10570-10572.

[22]林丛发,钟爱青,林云斌,等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1)：84-86.

[23]苏钛,张晓南.液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究[J].中国野生植物资源,2009,28(4)：54-56.

[24]韦晓新,苏江,何铁光.因素对铁皮石斛原球茎生长和多糖积累的影响[J].广西农业科学,2008（9）：601-606.

[25]何铁光,杨丽涛,李杨瑞,等.蔗糖对铁皮石斛原球茎生长与多糖积累的影响[J].安徽农业科学,2007,19(1)：84-86,91.

[26]贾薇,唐庆九.硫酸苯酚法测定云芝多糖含量的效果[J].河南农业科学,1999（8）：24-25.

[27]杨正坤,王秀丽等.考马斯亮蓝染色法测定大豆茎叶中蛋白质含量[J].湖北农业科学,2012（10）：4610-4613.

[28]宋经元,郭顺星,肖培根.氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响[J].云南植物研究,2008,30(1)：105-109.

[29]高洪波,李敬蕊,章铁军,等.甘氨酸和谷氨酸与钼配施对生菜品质的影响[J].西北植物学报,2010,30(5)：968-973.

[30]荆留萍,杜双田,金凌云,等.8种物质对蛹虫草液体发酵中虫草素及多糖含量的影响[J].西北农林科技大学学报,2010,38(11)：156-160.

[31]Flowers T J.Salt tolerance in Suaeda maritima (L.) Dum：the effect of sodium chloride on growth,respiration,and soluble enzymes in a comparative study with Pisum sativumL[J].J Exp Bot,1972,23：310-321.

[32]李宗菊,桂明英.谷氨酸在无糖组织培养中的应用[J].西南农业学报,1999,12(3)：45-49.


Memo

Memo:
-
Last Update: 2014-08-13