Study on in vitro Culture of Bulblets Regeneration Derived From Bulb Scales of Tulipa edulis (Miq.) Bak.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on in vitro Culture of Bulblets Regeneration Derived From Bulb Scales of Tulipa edulis (Miq.) Bak.

Author(s):: SUN Jun-wei; ZHU Cheng; WANG Fei-juan; JIANG Qiong; DING Yan-fei; College of Life Sciences,China Jiliang University,Hangzhou,Zhejiang 310018

Keywords:: Tulipa edulis (Miq.) Bak.; bulb scale; regenerated bulblets; plant growth regulator

Abstract:: Taking the bulb scales as explants,the effects of different treatments on in vitro regeneration of bulblet derived from different parts of bulb scale of Tulipa edulis (Miq.) Bak.were studied.The results showed that outer bulb scales was better than middle and inner bulb scales；when the convexity of bulb scale was upward,higher bulblets induction rate and more bulblets number were recorded.Although many media could induce bulblets,the best media was 1/2MS+TDZ 0.3 mg/L+6-BA 2.0 mg/L,with 93.3% of the induction rate and 12.21 of bulblets numbers；the most appropriate mdium for the rooting and transplanting was 1/4MS+NAA 0.1 mg/L+IAA 0.3 mg/L,with 97% of rooting rate and 98% of transplanting rate.

[1]国家中医药管理局《中华本草》编委会.中华本草[M].上海：上海科学技术出版社，1999.

[2]阮小丽,施大文.山慈菇的抗肿瘤及抑菌作用[J].中药材,2009,32(12)：1886-1888.

[3]吴正军,朱再标,郭巧生,等.老鸦瓣种子生理及其萌发特性研究[J].中国中药杂志,2012,37(5)：575-579.

[4]吴正军,朱再标,郭巧生,等.老鸦瓣传粉生物学初步研究[J].中国中药杂志,2012,37(3)：293-297.

[5]杨永刚,代汉萍,胡新颖,等.郁金香器官离体培养再生小鳞茎的研究[J].园艺学报,2006,33(5)：1133-1136.

[6]邴其忠.光慈菇引种栽培及离体培养技术[D].北京：中国协和医学大学，2008.

[7]毛洪玉,王瑛,刘迪,等.郁金香离体快繁技术研究[J].北方园艺,2012(14)：114-118.

[8]龚明霞,何铁光,方锋学,等.郁金香鳞片离体培养再生小鳞茎研究[J].广西农业科学,2010,41(11)：1158-1160.

Last Update: 2014-08-13